Interactions of nucleic acids with distamycins. Binding of Dst-3 to d(CGTTTAAACG) sub(2) and d(CGTACGTACG) sub(2)

The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG) sub(2) and d(CGTACGTACG) sub(2) was investigated by two independent techniques: UV-Vis absorption in the Job's plot approach and induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with c...

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Bibliographic Details
Published in:Nucleic acids research 1991-01, Vol.19 (7), p.1695-1698
Main Authors: Capobianco, M L, Colonna, F P, ni, A, Garbesi, A, Iotti, S, Moretti, I, Samori, B, Tondelli, L
Format: Article
Language:English
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Summary:The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG) sub(2) and d(CGTACGTACG) sub(2) was investigated by two independent techniques: UV-Vis absorption in the Job's plot approach and induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall affinities. This result indicates that a row of six A:T base pairs can accommodate two molecules of drug and that the minimal binding site of Distamycin 3 can consist of just two A:T base pairs.
ISSN:0305-1048