Bacillus cereus may produce two or more diarrheal enterotoxins

Abstract Bacillus cereus strains were tested for production of diarrheal enterotoxin by the reverse passive latex agglutination test and for presence of B. cereus enterotoxin gene (bceT) by polymerase chain reaction. About 50% of 56 B. cereus strains reacted positive in broth culture in the reverse...

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Bibliographic Details
Published in:FEMS microbiology letters 1997-04, Vol.149 (2), p.245-248
Main Authors: Ombui, J.N. (Nairobi Univ. (Kenya). Faculty of Veterinary Medicine, Dept. of Public Health, Pharmacology and Toxicology), Schmieger, H, Kagiko, M.M, Arimi, S.M
Format: Article
Language:English
Subjects:
Agglutination
BACILLUS CEREUS
Bacillus cereus - genetics
Bacillus cereus - metabolism
Bacterial Proteins - genetics
Diarrhea
Diarrhea - microbiology
DNA, Bacterial - analysis
Enterotoxin
ENTEROTOXINAS
ENTEROTOXINE
ENTEROTOXINS
Enterotoxins - genetics
Enterotoxins - metabolism
Food contamination
Food poisoning
Foodborne Diseases - microbiology
Hybridization
Hydrolysis
Latex
Latex agglutination
Microbiology
PCR
Polymerase Chain Reaction
Reaction products
Starch - metabolism
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Summary:Abstract Bacillus cereus strains were tested for production of diarrheal enterotoxin by the reverse passive latex agglutination test and for presence of B. cereus enterotoxin gene (bceT) by polymerase chain reaction. About 50% of 56 B. cereus strains reacted positive in broth culture in the reverse passive latex agglutination test, while the bceT gene was detected in 41.1%. Sixteen percent of the strains were positive for both diarrheal enterotoxin and bceT gene. A 741 bp probe prepared from the polymerase chain reaction product detected bceT gene in all strains that were positive with the polymerase chain reaction. This study indicates a likelihood of two or more enterotoxins being produced by B. cereus which may be involved in causing diarrheal type food poisoning.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1997.tb10336.x