Cryoalgotox: Use of cryopreserved alga in a semistatic microplate test

Use of cryopreserved alga Selenastrum capricornutum has been evaluated as a simple and cost‐efficient procedure in a new semistatic algal ecotoxicity test. Experiments have been conducted to compare performance criteria of this method, named Cryoalgotox, versus the classic microplate test using fres...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 1997-03, Vol.16 (3), p.505-508
Main Authors: Benhra, Ali, Radetski, Claudemir Marcos, Férard, Jean-François
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Cryopreservation
Ecotoxicology
Ecotoxicology, biological effects of pollution
Environmental pollutants toxicology
Freshwater
Fundamental and applied biological sciences. Psychology
General aspects
Medical sciences
Microbiotest
Selenastrum capricornutum
Techniques
Toxicology
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Summary:Use of cryopreserved alga Selenastrum capricornutum has been evaluated as a simple and cost‐efficient procedure in a new semistatic algal ecotoxicity test. Experiments have been conducted to compare performance criteria of this method, named Cryoalgotox, versus the classic microplate test using fresh algae. Cryoalgotox 72‐h 50% effective concentrations (EC50s) determined with Cd2+, Cu2+, Cr6+, and atrazine were more sensitive, repeatable (low coefficients of variation), and reproducible (low time effect) than the results obtained with the classical microplate tests. The effect of storage time at −80°C on the sensitivity of the algae was assessed using cadmium as a toxic reference; it was shown that algae stored at −80°C over a 3‐month period gave comparable toxicity results to those found with fresh algae.
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.5620160316