Fragile algae : axenic culture of field-collected samples of Heterosigma carterae

Twelve cultures of the naturally wall-less, unicellular chromophyte Heterosigma carterae originating from North America and Japan were analyzed. Several of these had been recovered from blooms that were toxic to fish. Algal cultures were concentrated by centrifugation onto a Percoll pad. The cells w...

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Bibliographic Details
Published in:Marine biology 1996-04, Vol.125 (2), p.421-426
Main Authors: CONNELL, L, CATTOLICO, R. A
Format: Article
Language:English
Subjects:
Algae
Animal, plant and microbial ecology
Antibiotics
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects. Techniques
Heterosigma carterae
Marine
Marine biology
Methods and techniques (sampling, tagging, trapping, modelling...)
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Summary:Twelve cultures of the naturally wall-less, unicellular chromophyte Heterosigma carterae originating from North America and Japan were analyzed. Several of these had been recovered from blooms that were toxic to fish. Algal cultures were concentrated by centrifugation onto a Percoll pad. The cells were selectively treated with a cascade of antibiotics, including ampicillin, kanamycin, neomycin, nitrofuratoin, penicillin-G, rifampin, and streptomycin. Each isolate was treated with a specific subset of the antibiotics to attain axenicity. Doubling times of the individual isolates varied from 1.8 to 2.9 d, and were not influenced by previous antibiotic treatment. The studies were conducted from 1991-1994 at the University of Washington, Seattle, Washington, USA.
ISSN:0025-3162
1432-1793
DOI:10.1007/BF00346322