Coupled reductive and oxidative degradation of 4-chloro-2-nitrophenol by a co-immobilized mixed culture system

The restriction of oxygen transfer in Ca-alginate beads used for the immobilization of microbial cells was applied to a coupled reductive and oxidative microbial degradation of the xenobiotic 4-chloro-2-nitrophenol (CNP). The conversion of CNP by Enterobacter cloacae under anaerobic conditions led t...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1990-10, Vol.34 (1), p.108-115
Main Authors: BEUNINK, J, REHM, H.-J
Format: Article
Language:English
Subjects:
4-chloro-2-nitrophenol
Aerobiosis
Alcaligenes
Alcaligenes - metabolism
Anaerobiosis
biodegradation
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Enterobacter - metabolism
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
immobilized cells
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microspheres
Nitrophenols - metabolism
Oxidation-Reduction
Spectrophotometry, Infrared
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Summary:The restriction of oxygen transfer in Ca-alginate beads used for the immobilization of microbial cells was applied to a coupled reductive and oxidative microbial degradation of the xenobiotic 4-chloro-2-nitrophenol (CNP). The conversion of CNP by Enterobacter cloacae under anaerobic conditions led to the formation of 4-chloro-2-aminophenol (CAP, 81%) and 4-chloro-2-acetaminophenol (CAAP, 16%) after 50 h incubation. CAP, the main reduction product, was further degraded under aerobic conditions by Alcaligenes sp. TK-2, a hybrid strain isolated by conjugative in-vivo gene transfer. Whereas both degradation steps excluded one another in homogeneous systems with free cells, a coupled reductive and oxidative degradation of CNP was observed in one aerated reactor system after co-immobilization of both strains in Ca alginate. The diameter of the alginate beads used for immobilization was recognized as one main factor determining the properties of this mixed culture system.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00170933