Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts

Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzym...

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Bibliographic Details
Published in:Biotechnology techniques 1996-10, Vol.10 (10), p.717-720
Main Authors: Kim, Y.S, Hara, M, Ikebukuro, K, Miyake, J, Ohkawa, H, Karube, I
Format: Article
Language:English
Subjects:
7-ethoxycoumarin
7-hydroxycoumarin
Biological and medical sciences
Biotechnology
chemical reactions
chloroplasts
Clostridium pasteurianum
coumarins
cytochrome P-450
de-ethylation
electron transfer
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
microsomes
Miscellaneous
NADP (coenzyme)
photosynthesis
photosystem I
photosystem II
rats
recombinant proteins
reduction
Saccharomyces cerevisiae
Spinacia oleracea
unspecific monooxygenase
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Summary:Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzyme to convert 7-ethoxycoumarin to 7-hydroxycoumarin.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00222553