A Bacillus subtilis locus encoding several gene products affecting transport of cations

A 3.6-kb DNA fragment from Bacillus subtilis was found to complement the K + uptake-deficient Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K +. Insertional mutagenesis and subclon...

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Bibliographic Details
Published in:Gene 1997-03, Vol.188 (1), p.91-94
Main Authors: Sturr, Michael G., Ablooglu, Ararat J., Krulwich, Terry A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
Biological Transport
Cations - metabolism
Chromosome Mapping
Cloning, Molecular
Complementation
CzcD
DNA, Bacterial
Escherichia coli
Escherichia coli - genetics
Genes, Bacterial
Genetic Complementation Test
LysR
Molecular Sequence Data
Open Reading Frames
Potassium - metabolism
Potassium uptake
Sodium - metabolism
Sodium resistance
TrkA
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Summary:A 3.6-kb DNA fragment from Bacillus subtilis was found to complement the K + uptake-deficient Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K +. Insertional mutagenesis and subcloning identified a single gene responsible for the complementation. This gene coded for an apparent homolog of E. coli TrkA. Sequence analysis of the cloned region also revealed three additional open reading frames. These included: a gene encoding a homolog to the czcD gene product of Alcaligenes eutrophus, a lysR-type regulatory gene which was found to enhance Na + resistance in E. coli NM81 (Δ nhaA) in a separate complementation test, and an orfD with no significant similarity to sequences deposited in Genbank.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00784-6