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A Bacillus subtilis locus encoding several gene products affecting transport of cations
A 3.6-kb DNA fragment from Bacillus subtilis was found to complement the K + uptake-deficient Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K +. Insertional mutagenesis and subclon...
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| Published in: | Gene 1997-03, Vol.188 (1), p.91-94 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c391t-ee77f6eebace475db1a5dc66288fcc7e9e66eeac4efb8515fdff9c42074bdc243 |
| container_end_page | 94 |
| container_issue | 1 |
| container_start_page | 91 |
| container_title | Gene |
| container_volume | 188 |
| creator | Sturr, Michael G. Ablooglu, Ararat J. Krulwich, Terry A. |
| description | A 3.6-kb DNA fragment from
Bacillus subtilis was found to complement the K
+ uptake-deficient
Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K
+. Insertional mutagenesis and subcloning identified a single gene responsible for the complementation. This gene coded for an apparent homolog of
E. coli TrkA. Sequence analysis of the cloned region also revealed three additional open reading frames. These included: a gene encoding a homolog to the
czcD gene product of
Alcaligenes eutrophus, a
lysR-type regulatory gene which was found to enhance Na
+ resistance in
E. coli NM81 (Δ
nhaA) in a separate complementation test, and an
orfD with no significant similarity to sequences deposited in Genbank. |
| doi_str_mv | 10.1016/S0378-1119(96)00784-6 |
| format | article |
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Bacillus subtilis was found to complement the K
+ uptake-deficient
Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K
+. Insertional mutagenesis and subcloning identified a single gene responsible for the complementation. This gene coded for an apparent homolog of
E. coli TrkA. Sequence analysis of the cloned region also revealed three additional open reading frames. These included: a gene encoding a homolog to the
czcD gene product of
Alcaligenes eutrophus, a
lysR-type regulatory gene which was found to enhance Na
+ resistance in
E. coli NM81 (Δ
nhaA) in a separate complementation test, and an
orfD with no significant similarity to sequences deposited in Genbank.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(96)00784-6</identifier><identifier>PMID: 9099864</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Bacillus subtilis ; Bacillus subtilis - genetics ; Bacillus subtilis - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Biological Transport ; Cations - metabolism ; Chromosome Mapping ; Cloning, Molecular ; Complementation ; CzcD ; DNA, Bacterial ; Escherichia coli ; Escherichia coli - genetics ; Genes, Bacterial ; Genetic Complementation Test ; LysR ; Molecular Sequence Data ; Open Reading Frames ; Potassium - metabolism ; Potassium uptake ; Sodium - metabolism ; Sodium resistance ; TrkA</subject><ispartof>Gene, 1997-03, Vol.188 (1), p.91-94</ispartof><rights>1997 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-ee77f6eebace475db1a5dc66288fcc7e9e66eeac4efb8515fdff9c42074bdc243</citedby><cites>FETCH-LOGICAL-c391t-ee77f6eebace475db1a5dc66288fcc7e9e66eeac4efb8515fdff9c42074bdc243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9099864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sturr, Michael G.</creatorcontrib><creatorcontrib>Ablooglu, Ararat J.</creatorcontrib><creatorcontrib>Krulwich, Terry A.</creatorcontrib><title>A Bacillus subtilis locus encoding several gene products affecting transport of cations</title><title>Gene</title><addtitle>Gene</addtitle><description>A 3.6-kb DNA fragment from
Bacillus subtilis was found to complement the K
+ uptake-deficient
Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K
+. Insertional mutagenesis and subcloning identified a single gene responsible for the complementation. This gene coded for an apparent homolog of
E. coli TrkA. Sequence analysis of the cloned region also revealed three additional open reading frames. These included: a gene encoding a homolog to the
czcD gene product of
Alcaligenes eutrophus, a
lysR-type regulatory gene which was found to enhance Na
+ resistance in
E. coli NM81 (Δ
nhaA) in a separate complementation test, and an
orfD with no significant similarity to sequences deposited in Genbank.</description><subject>Amino Acid Sequence</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacillus subtilis - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Biological Transport</subject><subject>Cations - metabolism</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Complementation</subject><subject>CzcD</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>LysR</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Potassium - metabolism</subject><subject>Potassium uptake</subject><subject>Sodium - metabolism</subject><subject>Sodium resistance</subject><subject>TrkA</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkEtLxDAQx4Mouj4-gpCT6KGabNukOYkuvmDBg4rHkE4mEuk2a9IKfnuzD7w6l2H4_-f1I-SUs0vOuLh6YaVsCs65OlfigjHZVIXYIRPeSFUwVja7ZPJnOSCHKX2yHHU93Sf7iinViGpC3m_orQHfdWOiaWwH3_lEuwC5xB6C9f0HTfiN0XT0A3ukyxjsCEOixjmEYaUP0fRpGeJAg6NgBh_6dEz2nOkSnmzzEXm7v3udPRbz54en2c28gFLxoUCU0gnE1gBWsrYtN7UFIaZN4wAkKhRZNVCha5ua1846p6CaMlm1FqZVeUTONnPzXV8jpkEvfALsOtNjGJPmtaqYVGU21hsjxJBSRKeX0S9M_NGc6RVQvQaqV7S0EnoNVIvcd7pdMLYLtH9dW4JZv97omL_89hh1Ap_RofUx89E2-H82_AKdqohN</recordid><startdate>19970325</startdate><enddate>19970325</enddate><creator>Sturr, Michael G.</creator><creator>Ablooglu, Ararat J.</creator><creator>Krulwich, Terry A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19970325</creationdate><title>A Bacillus subtilis locus encoding several gene products affecting transport of cations</title><author>Sturr, Michael G. ; Ablooglu, Ararat J. ; Krulwich, Terry A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-ee77f6eebace475db1a5dc66288fcc7e9e66eeac4efb8515fdff9c42074bdc243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacillus subtilis - metabolism</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Biological Transport</topic><topic>Cations - metabolism</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>Complementation</topic><topic>CzcD</topic><topic>DNA, Bacterial</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>LysR</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Potassium - metabolism</topic><topic>Potassium uptake</topic><topic>Sodium - metabolism</topic><topic>Sodium resistance</topic><topic>TrkA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sturr, Michael G.</creatorcontrib><creatorcontrib>Ablooglu, Ararat J.</creatorcontrib><creatorcontrib>Krulwich, Terry A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sturr, Michael G.</au><au>Ablooglu, Ararat J.</au><au>Krulwich, Terry A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Bacillus subtilis locus encoding several gene products affecting transport of cations</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1997-03-25</date><risdate>1997</risdate><volume>188</volume><issue>1</issue><spage>91</spage><epage>94</epage><pages>91-94</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A 3.6-kb DNA fragment from
Bacillus subtilis was found to complement the K
+ uptake-deficient
Escherichia coli strain TK2420. Transformation with a pKLO61 plasmid harboring this fragment conferred the capacity to grow on a minimal medium containing only 10 mM K
+. Insertional mutagenesis and subcloning identified a single gene responsible for the complementation. This gene coded for an apparent homolog of
E. coli TrkA. Sequence analysis of the cloned region also revealed three additional open reading frames. These included: a gene encoding a homolog to the
czcD gene product of
Alcaligenes eutrophus, a
lysR-type regulatory gene which was found to enhance Na
+ resistance in
E. coli NM81 (Δ
nhaA) in a separate complementation test, and an
orfD with no significant similarity to sequences deposited in Genbank.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9099864</pmid><doi>10.1016/S0378-1119(96)00784-6</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1997-03, Vol.188 (1), p.91-94 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15940793 |
| source | Elsevier |
| subjects | Amino Acid Sequence Bacillus subtilis Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biological Transport Cations - metabolism Chromosome Mapping Cloning, Molecular Complementation CzcD DNA, Bacterial Escherichia coli Escherichia coli - genetics Genes, Bacterial Genetic Complementation Test LysR Molecular Sequence Data Open Reading Frames Potassium - metabolism Potassium uptake Sodium - metabolism Sodium resistance TrkA |
| title | A Bacillus subtilis locus encoding several gene products affecting transport of cations |
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