Quantitative Determination of Thiabendazole in Fruit Juices and Bulk Juice Concentrates Using a Thiabendazole Monoclonal Antibody

A thiabendazole [2-(4-thiazolyl)-1H-benzimidazole, TBZ] monoclonal antibody has been employed in an enzyme immunoassay (EIA) procedure to analyze TBZ in fruit juices and bulk juice concentrates. Samples were prepared by partitioning into methylene chloride and analyzed by EIA and HPLC. The average r...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1995-05, Vol.43 (5), p.1407-1412
Main Authors: Bushway, Rodney J, Brandon, David L, Bates, Anne H, Li, Lei, Larkin, Karen A, Young, Barbara S
Format: Article
Language:English
Subjects:
ALIMENT CONCENTRE POUR ANIMAUX
ALIMENTOS
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Biological and medical sciences
CONTAMINACION
CONTAMINATION
FONGICIDE
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
FUNGICIDAS
JUGO DE FRUTAS
JUS DE FRUITS
PIENSOS CONCENTRADOS
PRODUIT ALIMENTAIRE
RESIDU
RESIDUOS
TECHNIQUE IMMUNOENZYMATIQUE
TECNICAS INMUNOENZIMATICAS
THIABENDAZOLE
TIABENDAZOL
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A thiabendazole [2-(4-thiazolyl)-1H-benzimidazole, TBZ] monoclonal antibody has been employed in an enzyme immunoassay (EIA) procedure to analyze TBZ in fruit juices and bulk juice concentrates. Samples were prepared by partitioning into methylene chloride and analyzed by EIA and HPLC. The average recovery for 50 juices and concentrates spiked at 5-1000 ppb was 93%. Forty-one market samples containing TBZ in the range from 0.6 to 280 ppb were analyzed, and the analyses by the two methods showed excellent agreement (r = 0.988). The detection limits were 0.5 ppb for juice and 2 ppb for bulk concentrates. Because a quicker and simpler sample preparation scheme would be desirable, seven positive juice samples were analyzed following direct dilution. For these samples, the correlation between EIA and HPLC was still very good (r = 0.92). Bulk juice concentrates generally required partitioning to completely eliminate matrix effects
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00053a051