Subcellular localization of blood group A substance produced by pancreatic adenocarcinoma induced in hamsters by N-nitrosobis(2-oxopropyl)amine (BOP) and by its cell line (PC-1)

• Published in: Carcinogenesis (New York) 1991-03, Vol.12 (3), p.509-514
• Main Authors: Egami, Hiroshi, Chaney, William G., Takiyama, Yoshiyuki, Pour, Parviz M.
• Format: Article
• Language: English
• Subjects: ABO Blood-Group System; Adenocarcinoma - chemically induced; Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - metabolism; Antigens, Differentiation - immunology; Antigens, Differentiation - metabolism; blood group A; Blotting, Western; CD11 Antigens; CD18 Antigens; Cell Membrane - immunology; Cell Membrane - metabolism; Cricetinae; Duodenum - immunology; Duodenum - metabolism; Electrophoresis, Polyacrylamide Gel - methods; Humans; Immunohistochemistry; Injections, Subcutaneous; Microscopy, Electron - methods; Neoplasm Transplantation; Nitrosamines; Pancreas - immunology; Pancreas - metabolism; Pancreatic Neoplasms - chemically induced; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Receptors, Leukocyte-Adhesion - immunology; Receptors, Leukocyte-Adhesion - metabolism; Sodium Dodecyl Sulfate; Subcellular Fractions - metabolism; Tumor Cells, Cultured
• ISSN: 0143-3334; 1460-2180
• DOI: 10.1093/carcin/12.3.509

The subcellular localization and biochemical characteristics of blood group A antigen were studied by immunogold methods and by SDS-PAGE and Western blotting procedures in N-nitrosobis(2-oxopropyl)amine (BOP)-induced pancreatic cancer (PC) in Syrian hamsters, in the pancreatic cancer cell line (PC-1) derived from a primary induced pancreatic cancer, and in intrapancreatic and subcutaneous transplants of PC-1 cells. Normal hamster duodenal epithelial cells expressing A antigen were compared with the normal hamster pancreas (lacking A antigen), human PC tissues from patients with blood group A and human PC cell lines. Blood group A antigen was present on the membrane of hamster duodenal cells, but was absent in the normal pancreatic cells. A antigen was localized mainly on the cell membrane of the hamster cancer cells both in vivo and in vitro. Glycoproteins with blood group A specificity were observed by SDS–PAGE and Western blotting procedures in the membrane fraction of PC-1 cells, with a major component of molecular mass of ∽ 120 kd. Similar migration patterns were observed in the primary induced PC and in subcutaneous and intrapancreatic transplants of PC-1 cells. Membrane preparations from cell lines derived from two primary pancreatic cancers from patients of blood group A and from human pancreatic cell lines, CD11 and CD18, showed a major A reactive component with a molecular mass similar to that found in the hamster PC cells. These findings suggest that: (i) both the hamster and human PC cells in vitro produce glycoproteins with blood group A specificity of similar molecular masses; (ii) differences exist in the structure of the glycoprotein immunoreactive with the anti-A antigen between the normal and cancerous cells; and (iii) differences exist in the molecular mass of the anti-A reactive substance between hamsters and human PC cells and between tissues in vivo and in vitro.