Determination of chlorothalonil in water and agricultural products by a magnetic particle-based enzyme immunoassay

A competitive enzyme immunoassay for the quantitation of chlorothalonil in water and agricultural products was developed. This immunoassay utilizes amine-terminated superparamagnetic particles as the solid phase to covalently attach polyclonal rabbit anti-chlorothalonil antisera. Specificity studies...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1995-05, Vol.43 (5), p.1413-1419
Main Authors: Lawruk, Timothy S, Gueco, Adrian M, Jourdan, Scott W, Scutellaro, Adele M, Fleeker, James R, Herzog, David P, Rubio, Fernando M
Format: Article
Language:English
Subjects:
AGUA POTABLE
AGUA SUPERFICIAL
ALIMENTOS
APIO
Biological and medical sciences
CAMPO MAGNETICO
CELERI
CHAMP MAGNETIQUE
CHLOROTHALONIL
CLOROTALONIL
CONTAMINACION
CONTAMINATION
EAU POTABLE
EAU SUPERFICIELLE
FONGICIDE
FRUITS
FRUTAS
Fundamental and applied biological sciences. Psychology
FUNGICIDAS
GUISANTE
IMMUNSERUM
Methods and techniques. Experimental designs. Plant protection equipments
Phytopathology. Animal pests. Plant and forest protection
POIS
POLLUTION DE L'EAU
POLUCION DEL AGUA
PRODUIT ALIMENTAIRE
RESIDU
RESIDUOS
SUERO INMUNE
TECHNIQUE IMMUNOENZYMATIQUE
TECNICAS INMUNOENZIMATICAS
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Description
Summary:A competitive enzyme immunoassay for the quantitation of chlorothalonil in water and agricultural products was developed. This immunoassay utilizes amine-terminated superparamagnetic particles as the solid phase to covalently attach polyclonal rabbit anti-chlorothalonil antisera. Specificity studies indicate that the antibody can distinguish chlorothalonil from its major metabolites and structurally similar pesticides. The immunoassay has a detection limit of 0.07 parts per billion (ppb, ng/mL) in water. The assay compares favorably with GC measurements when water samples are analyzed (r = 0.984). Studies indicate that the assay can potentially be used to analyze agricultural products after homogenization with acetone (detection limit of 20 ppb for celery) or to analyze dislodgable residues after a methanol surface wash (detection limit of 5 ppb for snow pea pods)
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00053a052