Molecular diagnostics of three Diabrotica (Coleoptera: Chrysomelidae) pest species

A 257 bp region of the mitochondrial ND4 gene was analyzed for genetic variation in three species of corn rootworm, southern corn rootworm (Diabrotica undecimpunctata howardi Barber, SCR), northern corn rootworm (D. barberi Smith and Lawrence, NCR), and western corn rootworm (D. virgifera virgifera...

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Bibliographic Details
Published in:Journal of the Kansas Entomological Society 1996-07, Vol.69 (3), p.260-266
Main Authors: Szalanski, A.L. (University of Nebraska, Lincoln, NE.), Powers, T.O
Format: Article
Language:English
Subjects:
ADN
BIOLOGICAL DIFFERENCES
Biological taxonomies
CHEMOTAXONOMY
CHIMIOTAXONOMIE
Chrysomelidae
Coleoptera
DIABROTICA BARBERI
DIABROTICA UNDECIMPUNCTATA
DIABROTICA UNDECIMPUNCTATA HOWARDI
Diabrotica virgifera
DIABROTICA VIRGIFERA VIRGIFERA
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
DISTANCE GENETIQUE
DISTANCIA GENETICA
DNA
Entomology
Enzymes
genban/u63088
genbank/u63087
genbank/u63089
GENE
GENES
GENETIC DISTANCE
GENETIC POLYMORPHISM
GENETICA
GENETICS
GENETIQUE
Insect larvae
INSECTE NUISIBLE
INSECTOS DANINOS
MITOCHONDRIA
Mitochondrial DNA
MITOCHONDRIE
MITOCONDRIA
molecular sequence data
nd4 gene
NUCLEASAS
NUCLEASE
NUCLEASES
NUCLEOTIDE SEQUENCE
PCR
PEST INSECTS
Pests
POLIMORFISMO GENETICO
POLYMERASE CHAIN REACTION
POLYMORPHISME GENETIQUE
QUIMIOTAXONOMIA
RESTRICTION ENDONUCLEASES
RESTRICTION MAPPING
RFLP
Rootworms
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
SPECIES DIFFERENCES
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Summary:A 257 bp region of the mitochondrial ND4 gene was analyzed for genetic variation in three species of corn rootworm, southern corn rootworm (Diabrotica undecimpunctata howardi Barber, SCR), northern corn rootworm (D. barberi Smith and Lawrence, NCR), and western corn rootworm (D. virgifera virgifera LeConte, WCR). Nucleotide sequencing revealed 26 polymorphic sites. Genetic distances averaged 8% for all pair-wise comparisons among the three species. Restriction maps were constructed from sequence data and compared to potential species specific restriction sites. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) revealed three restriction enzymes (Alu I, Apo I and Sau 3A) which produced diagnostic patterns for both adults and larvae. Only NCR showed intraspecific polymorphism.
ISSN:0022-8567
1937-2353