Low-temperature accumulation of alcohol dehydrogenase-1 mRNA and protein activity in maize and rice seedlings

Low-temperature stress was shown to cause a rapid increase in steady-state levels of alcohol dehydrogenase-1 message (Adh1) and protein activity (ADH1) in maize (Zea mays) (B37N, A188) and rice (Oryza sativa) (Taipei 309, Calmochi 101) seedlings. Maize roots and rice shoots and roots from 7-day seed...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-03, Vol.95 (3), p.699-706
Main Authors: Christie, P.J. (Stanford University, Stanford, CA), Hahn, M, Walbot, V
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ALCOHOL DESHIDROGENASA
Alcohols
ALCOOL DESHYDROGENASE
Ambient temperature
ARN MENSAJERO
ARN MESSAGER
Biological and medical sciences
Corn
Enzymes
ESTRES
EXPRESION GENICA
EXPRESSION DES GENES
FRIO
FROID
Fundamental and applied biological sciences. Psychology
Gels
Genes
Messenger RNA
Metabolism
METABOLISME
METABOLISMO
ORYZA SATIVA
Plant physiology and development
Plant tissues
Plants
PLANTULAS
PLANTULE
Rice
Seedlings
SINTESIS DE PROTEINAS
STRESS
SYNTHESE PROTEIQUE
ZEA MAYS
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Summary:Low-temperature stress was shown to cause a rapid increase in steady-state levels of alcohol dehydrogenase-1 message (Adh1) and protein activity (ADH1) in maize (Zea mays) (B37N, A188) and rice (Oryza sativa) (Taipei 309, Calmochi 101) seedlings. Maize roots and rice shoots and roots from 7-day seedlings shifted to low temperature (100 degrees C) contained as much as 15-fold more Adh1 mRNA and 8-fold more ADH1 protein activity than the corresponding tissues from untreated seedlings. Time-course studies showed that these tissues accumulated Adh1 mRNA and ADH1 activity severalfold within 4- to 8-hour, levels plateaued within 20 to 24 hours, and remained elevated at 4 days of cold treatment. Within 24 hours of returning cold-stressed seedlings to ambient temperature, Adh1 mRNA and ADH1 activity decreased to pretreatment levels. Histochemical staining of maize and rice tissue imprints showed that ADH activity was enhanced along the lengths of cold-stressed maize primary roots and rice roots, and along the stems and leaves of rice shoots. Our observations suggest that short-term cold stress induces Adh1 gene expression in certain plant tissues, which, reminiscient of the anaerobic response, may reflect a fundamental shift in energy metabolism to ensure tissue survival during the stress period
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.95.3.699