Generation of a Nested Series of Interstitial Deletions in Yeast Artificial Chromosomes Carrying Human DNA

We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-07, Vol.88 (13), p.5744-5748
Main Authors: Campbell, Colin, Gulati, Rajiv, Nandi, Asit K., Floy, Kimberly, Hieter, Philip, Kucherlapati, Raju S.
Format: Article
Language:English
Subjects:
550400 - Genetics
ANIMALS
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
CHROMOSOMAL ABERRATIONS
Chromosome Deletion
CHROMOSOMES
Cloning, Molecular - methods
Crossovers
deletion
DNA
DNA probes
ELECTROPHORESIS
Enzymes
EUMYCOTA
Exons
Fundamental and applied biological sciences. Psychology
FUNGI
GENE RECOMBINATION
Genes
Genetic Vectors
Genic rearrangement. Recombination. Transposable element
HETEROCHROMOSOMES
HUMAN X CHROMOSOME
Humans
In Vitro Techniques
MAMMALS
MAN
Mathematical vectors
MICROORGANISMS
Molecular and cellular biology
Molecular genetics
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
Plasmids
PRIMATES
Recombination, Genetic
Repetitive Sequences, Nucleic Acid
Restriction Mapping
RFLPS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
VERTEBRATES
X CHROMOSOME
X Chromosome - ultrastructure
YEASTS
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.13.5744