Induction of IP-10 chemokine promoter by measles virus: comparison with interferon- γ shows the use of the same response element but with differential DNA–protein binding profiles

Measles virus (MV) and interferon (IFN)- γ induced IP-10 chemokine mRNA in U373 glioblastoma cells. The minimal response element for both MV and IFN- γ was localized between nucleotide −231 and −153 of muIP-10 promoter, which contains an IFN-stimulated response element (ISRE) and the distal NF-κB d...

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Bibliographic Details
Published in:Journal of neuroimmunology 1997-07, Vol.77 (1), p.116-127
Main Authors: Nazar, Ahamed S.M.I, Cheng, Gaihua, Shin, Hyun S, Brothers, Paul N, Dhib-Jalbut, Suhayl, Shin, Moon L, Vanguri, Padmavathy
Format: Article
Language:English
Subjects:
Base Sequence
Chemokine CXCL10
Chemokines, CXC
Cytokines - genetics
Cytokines - immunology
DNA, Viral - immunology
DNA-Binding Proteins - genetics
DNA-Binding Proteins - immunology
Gene Deletion
Gene Expression Regulation, Neoplastic - immunology
Gene Expression Regulation, Viral - immunology
Glial cells
Glioblastoma
Humans
Interferon- γ
Interferon-gamma - immunology
IP-10 promoter
Measles virus
Measles virus - genetics
Measles virus - immunology
Molecular Sequence Data
Mutagenesis - immunology
NF-kappa B - genetics
NF-kappa B - immunology
Promoter Regions, Genetic - immunology
RNA, Messenger - metabolism
Tumor Cells, Cultured - immunology
Tumor Cells, Cultured - virology
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Summary:Measles virus (MV) and interferon (IFN)- γ induced IP-10 chemokine mRNA in U373 glioblastoma cells. The minimal response element for both MV and IFN- γ was localized between nucleotide −231 and −153 of muIP-10 promoter, which contains an IFN-stimulated response element (ISRE) and the distal NF-κB d site. Mutation of individual elements showed that ISRE and NF-κB d were required to function together. DNA–protein binding profiles with the minimal response element showed that IFN- γ induced a complex consisting of STAT1 while MV induced a complex consisting of p50 and p65 in the absence of new protein synthesis. IFN- γ and MV also induced IRF-1 DNA binding activity which persisted for longer time periods with IFN- γ stimulation. Despite the functional requirement of both ISRE and NF-κB d elements, different combinations of DNA binding factors are used in the induction of IP-10 by MV or IFN- γ.
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(97)00070-2