Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns

Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme beta-glucuronidase. The expression patterns of this marker were analysed at di...

Full description

Saved in:
Bibliographic Details
Published in:Molecular breeding 1996-01, Vol.2 (4), p.329-338
Main Authors: Burrus, M, Molinier, J, Himber, C, Hunold, R, Bronner, R, Rousselin, P, Hahne, G
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium tumefaciens
beta-glucuronidase
embryo (plant)
embryonic axes
gene expression
gene transfer
genetic transformation
Helianthus annuus
histochemistry
meristems
regenerative ability
reporter genes
shoot apices
shoot meristems
transgenic plants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme beta-glucuronidase. The expression patterns of this marker were analysed at different developmental stages of the regenerated shoots. The results are consistent with the hypothesis that transformed shoots originate from transformation events that have occurred within the existing meristems. Two of the resulting chimaeric plants have been analysed in detail, and some representative gene integration patterns are presented.
ISSN:1380-3743
1572-9788
DOI:10.1007/BF00437911