Specific deletion of a large segment of pRA500: a 3,5‐xylenol degradative plasmid

3,5‐Xylenol degradative plasmid, pRA500 (approximately 500 kilobase pairs, kb) carried by Pseudomonas putida NCIB 9869, also encodes resistance to inorganic mercuric ions (Hgr). Following growth of Ps. putida on benzoate, p‐cresol, p‐hydroxybenzoate or protocatechuate, the frequency of loss of the 3...

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Bibliographic Details
Published in:Letters in applied microbiology 1991-01, Vol.12 (6), p.216-220
Main Authors: Jain, R.K., Bayly, R.C., Skurray, R.A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic technics
Methods. Procedures. Technologies
Mutant screening
Prokaryotes
Pseudomonas putida
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Summary:3,5‐Xylenol degradative plasmid, pRA500 (approximately 500 kilobase pairs, kb) carried by Pseudomonas putida NCIB 9869, also encodes resistance to inorganic mercuric ions (Hgr). Following growth of Ps. putida on benzoate, p‐cresol, p‐hydroxybenzoate or protocatechuate, the frequency of loss of the 3,5‐xylenol phenotype was 75–90%. The deletion of a large segment of approximately 350 kb from pRA500, to give rise to archetypal plasmid designated pRA502, occurred in such 3,5‐xylenol‐negative derivatives. During the conjugational transfer of pRA500 following selection of transconjugants for Hgronly, frequency of loss of 3,5‐xylenol phenotype was approximately 60%. A high number of these 3,5‐xylenol‐negative transconjugants carried pRA502. A site‐specific deletion is suggested in the formation of archetypal plasmid pRA502 by growth of Ps. putida on the above compounds and during transfer of pRA500.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1991.tb00543.x