Immobilization of organic solvent-soluble lipase in nonaqueous conditions and properties of the immobilized enzymes

Lipid-coated Pseudomonas lipase was successfully immobilized by entrapping with hydrophobic, photo-crosslinkable, resin prepolymers under non-aqueous conditions. We studied the feasibility of using this new type of biocatalyst, which is an immobilized organic solvent-soluble enzyme, for enzymatic sy...

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Bibliographic Details
Published in:Journal of biotechnology 1996-12, Vol.52 (2), p.81-88
Main Authors: Fukunaga, Kimitoshi, Minamijima, Noboru, Sugimura, Yoshiaki, Zhongzhi Zhang, Nakao, Katsumi
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Enzymatic esterifications
Fundamental and applied biological sciences. Psychology
Immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
Lipid-coated enzyme
Methods. Procedures. Technologies
Photo-crosslinkable prepolymer
Pseudomonas
Pseudomonas lipase
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Summary:Lipid-coated Pseudomonas lipase was successfully immobilized by entrapping with hydrophobic, photo-crosslinkable, resin prepolymers under non-aqueous conditions. We studied the feasibility of using this new type of biocatalyst, which is an immobilized organic solvent-soluble enzyme, for enzymatic synthesis in organic solvent. Esterification of lauric acid with benzyl alcohol in benzene and transesterification between racemic sulcatol and isopropenyl acetate in different organic solvents were carried out with the immobilized lipid-coated lipase as the model reactions. In both reaction systems, the immobilized organic solvent-soluble enzyme was observed to have excellent activity and stability.
ISSN:0168-1656
1873-4863
DOI:10.1016/S0168-1656(96)01630-6