Liquid fluidized bed starter culture of Trichoderma reesei for cellulase production

A starter culture of Trichoderma reesei (Rut-C30) prepared in a liquid fluidized bed reactor (LFBR) gave better growth and greater cellulase production in submerged fermentation than a conventional shake flask inoculum. The LFBR starter was prepared by first coating T. reesei spores to 0.25 mm size...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1991-03, Vol.27 (3), p.195-204
Main Authors: Tengerdy, R.P. (Colorado State University, Fort Collins, CO), Rho, W.H, Mohagheghi, A
Format: Article
Language:English
Subjects:
BIOLOGICAL PRODUCTION
BIOREACTEUR
BIOREACTORS
BIORREACTORES
CELLULASE
CELULASA
CEREAL BYPRODUCTS
fluidized beds
MAIS
MAIZ
MAIZE
MAIZE COBS
SOUS PRODUIT DE CEREALES
spores
starter cultures
SUBPRODUCTOS DE CEREALES
TRICHODERMA
TRICHODERMA LONGIBRACHIATUM
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Summary:A starter culture of Trichoderma reesei (Rut-C30) prepared in a liquid fluidized bed reactor (LFBR) gave better growth and greater cellulase production in submerged fermentation than a conventional shake flask inoculum. The LFBR starter was prepared by first coating T. reesei spores to 0.25 mm size corncob (1.0 x 10 super(8)g super(-1)) in a medium containing 1.0% corncob, 0.5 gL super(-1) xylose and 0.1 gL super(-1) lactose in a balanced salt solution, then fluidizing the particles in the LFBR for 36 h to allow germination of the spores, and covering the particles with an approx 30 mu m thick biofilm. This biofilm that developed in constant adherence to the lignocellulosic carrier, apparently became well adapted to grow rapidly on insoluble cellulose substrates (Solca Floc), and had the enzymes of the cellulase complex induced for increased cellulase production.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02921535