Novel intranasal immunization techniques for antibody induction and protection of mice against gastric Helicobacter felis infection

Intranasal (i.n.) delivery of antigen can be highly effective for generating circulating and secretory antibody responses. Mice were immunized i.n. with two antigens, human IgA, and Helicobacter pylori urease in the presence or absence of mucosal adjuvant. To restrict antigen delivery to the upper a...

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Bibliographic Details
Published in:Vaccine 1997-03, Vol.15 (4), p.370-376
Main Authors: Weltzin, Richard, Kleanthous, Harold, Guirakhoo, Farshad, Monath, Thomas P., Lee, Cynthia K.
Format: Article
Language:English
Subjects:
adjuvant
Adjuvants, Immunologic - administration & dosage
Administration, Intranasal
Administration, Oral
Animals
Antibodies, Bacterial - biosynthesis
Bacterial Toxins - immunology
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Cholera Toxin - immunology
Enterotoxins - immunology
Escherichia coli - immunology
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Gastritis - prevention & control
Helicobacter
Helicobacter - genetics
Helicobacter - immunology
Helicobacter felis
Helicobacter Infections - prevention & control
Helicobacter pylori - enzymology
Helicobacter pylori - genetics
Helicobacter pylori - immunology
Humans
Immunization Schedule
intranasal immunization
Mice
Microbiology
Urease - administration & dosage
Urease - genetics
Urease - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - immunology
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Summary:Intranasal (i.n.) delivery of antigen can be highly effective for generating circulating and secretory antibody responses. Mice were immunized i.n. with two antigens, human IgA, and Helicobacter pylori urease in the presence or absence of mucosal adjuvant. To restrict antigen delivery to the upper airways, protein solutions were administered in a small volume without anesthesia. Repeated daily i.n. administration of antigen without adjuvant elicited high levels of specific IgG in serum and IgA in serum, saliva, and feces. Once weekly i.n. immunization with co-administration of cholera toxin or Escherichia coli heat-labile toxin as adjuvant elicited somewhat lower levels of antibody to urease. When challenged with Helicobacter felis, only mice immunized with urease in the presence of adjuvant were protected against gastric infection.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(97)00203-X