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Ochratoxin A Content of Human Sera Determined by a Sensitive ELISA
A sensitive, monoclonal antibody-based ELISA test was developed and used for quantitative determination of ochratoxin A (OA) in human sera. The measuring range of this test (without sample dilution) was 0.2–2.0 ng/mL, and the detection limit was 0.2 ng/mL. The OA concentrations of 355 sera samples v...
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Published in: | Journal of analytical toxicology 1997-01, Vol.21 (1), p.44-48 |
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container_title | Journal of analytical toxicology |
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creator | Solti, László Salamon, Ferenc Barna-Vetró, Ildikó Gyöngyösi, Ágnes Szabó, Erzsébet Wölfling, Anna |
description | A sensitive, monoclonal antibody-based ELISA test was developed and used for quantitative determination of ochratoxin A (OA) in human sera. The measuring range of this test (without sample dilution) was 0.2–2.0 ng/mL, and the detection limit was 0.2 ng/mL. The OA concentrations of 355 sera samples varied from |
doi_str_mv | 10.1093/jat/21.1.44 |
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The measuring range of this test (without sample dilution) was 0.2–2.0 ng/mL, and the detection limit was 0.2 ng/mL. The OA concentrations of 355 sera samples varied from <0.2 to 10 ng/mL OA, but 75% of the samples contained 0.2–1.0 ng/mL. This amount reflects a tolerable daily intake (TDI) value of toxin. However, in some cases (6.8%), more than 1.0 ng/mL OA was measured, which is probably a result of elevated intake of OA, which may even exceed the “virtually safe dose”. Our data indicate that, like in many other countries, OA is present in food or feed products available in Hungary, and in order to save the health of consumers, their regular control is desirable.</description><identifier>ISSN: 0146-4760</identifier><identifier>EISSN: 1945-2403</identifier><identifier>DOI: 10.1093/jat/21.1.44</identifier><identifier>PMID: 9013292</identifier><identifier>CODEN: JATOD3</identifier><language>eng</language><publisher>Niles, IL: Oxford University Press</publisher><subject>Antibodies, Monoclonal - analysis ; Biological and medical sciences ; Consumers ; Data processing ; Edible Grain - chemistry ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Food ; Food toxicology ; Humans ; Hungary ; Medical sciences ; Mycotoxins - blood ; Ochratoxin A ; Ochratoxins - blood ; Osteoarthritis ; Reproducibility of Results ; Sensitivity and Specificity ; Toxicology ; Toxins</subject><ispartof>Journal of analytical toxicology, 1997-01, Vol.21 (1), p.44-48</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-1d7cafca9842fb9c1b61011c3c1001a948ef06a986fe3d1a32b5f40aeeba2b723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2537031$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9013292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Solti, László</creatorcontrib><creatorcontrib>Salamon, Ferenc</creatorcontrib><creatorcontrib>Barna-Vetró, Ildikó</creatorcontrib><creatorcontrib>Gyöngyösi, Ágnes</creatorcontrib><creatorcontrib>Szabó, Erzsébet</creatorcontrib><creatorcontrib>Wölfling, Anna</creatorcontrib><title>Ochratoxin A Content of Human Sera Determined by a Sensitive ELISA</title><title>Journal of analytical toxicology</title><addtitle>Journal of Analytical Toxicology</addtitle><addtitle>Journal of Analytical Toxicology</addtitle><description>A sensitive, monoclonal antibody-based ELISA test was developed and used for quantitative determination of ochratoxin A (OA) in human sera. The measuring range of this test (without sample dilution) was 0.2–2.0 ng/mL, and the detection limit was 0.2 ng/mL. The OA concentrations of 355 sera samples varied from <0.2 to 10 ng/mL OA, but 75% of the samples contained 0.2–1.0 ng/mL. This amount reflects a tolerable daily intake (TDI) value of toxin. However, in some cases (6.8%), more than 1.0 ng/mL OA was measured, which is probably a result of elevated intake of OA, which may even exceed the “virtually safe dose”. Our data indicate that, like in many other countries, OA is present in food or feed products available in Hungary, and in order to save the health of consumers, their regular control is desirable.</description><subject>Antibodies, Monoclonal - analysis</subject><subject>Biological and medical sciences</subject><subject>Consumers</subject><subject>Data processing</subject><subject>Edible Grain - chemistry</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Food</subject><subject>Food toxicology</subject><subject>Humans</subject><subject>Hungary</subject><subject>Medical sciences</subject><subject>Mycotoxins - blood</subject><subject>Ochratoxin A</subject><subject>Ochratoxins - blood</subject><subject>Osteoarthritis</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Toxicology</subject><subject>Toxins</subject><issn>0146-4760</issn><issn>1945-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqF0M-LEzEUB_AgylpXT56FHEQEmW5efk1zrHXXWSnsoQriJbxJE5y1M9NNMrL73xttqTc9BfL98JL3JeQlsDkwIy5uMV9wmMNcykdkBkaqiksmHpMZA6krWWv2lDxL6ZYx0AstzsiZYSC44TPy_sZ9j5jH-26gS7oah-yHTMdAm6nHgW58RPrBZx_7bvBb2j5QLJdD6nL309PL9fVm-Zw8CbhL_sXxPCdfri4_r5pqffPxerVcV04uRK5gWzsMDs1C8tAaB60GBuCEg_IvNHLhA9Ml1sGLLaDgrQqSofct8rbm4py8Oczdx_Fu8inbvkvO73Y4-HFKFpQxApQq8O2_YSlNKiaULPTdgbo4phR9sPvY9RgfCvrjbCnXcrBg5W_96jh4anu_PdljmyV_fcwxOdyFiIPr0olxJWom4O8i47T_z3vVAXYp-_sTxfjD6lrUyjZfv1lmNo1umk_2SvwCJJqbDw</recordid><startdate>19970101</startdate><enddate>19970101</enddate><creator>Solti, László</creator><creator>Salamon, Ferenc</creator><creator>Barna-Vetró, Ildikó</creator><creator>Gyöngyösi, Ágnes</creator><creator>Szabó, Erzsébet</creator><creator>Wölfling, Anna</creator><general>Oxford University Press</general><general>Preston</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>19970101</creationdate><title>Ochratoxin A Content of Human Sera Determined by a Sensitive ELISA</title><author>Solti, László ; Salamon, Ferenc ; Barna-Vetró, Ildikó ; Gyöngyösi, Ágnes ; Szabó, Erzsébet ; Wölfling, Anna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-1d7cafca9842fb9c1b61011c3c1001a948ef06a986fe3d1a32b5f40aeeba2b723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Antibodies, Monoclonal - analysis</topic><topic>Biological and medical sciences</topic><topic>Consumers</topic><topic>Data processing</topic><topic>Edible Grain - chemistry</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Food</topic><topic>Food toxicology</topic><topic>Humans</topic><topic>Hungary</topic><topic>Medical sciences</topic><topic>Mycotoxins - blood</topic><topic>Ochratoxin A</topic><topic>Ochratoxins - blood</topic><topic>Osteoarthritis</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Toxicology</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Solti, László</creatorcontrib><creatorcontrib>Salamon, Ferenc</creatorcontrib><creatorcontrib>Barna-Vetró, Ildikó</creatorcontrib><creatorcontrib>Gyöngyösi, Ágnes</creatorcontrib><creatorcontrib>Szabó, Erzsébet</creatorcontrib><creatorcontrib>Wölfling, Anna</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of analytical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Solti, László</au><au>Salamon, Ferenc</au><au>Barna-Vetró, Ildikó</au><au>Gyöngyösi, Ágnes</au><au>Szabó, Erzsébet</au><au>Wölfling, Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ochratoxin A Content of Human Sera Determined by a Sensitive ELISA</atitle><jtitle>Journal of analytical toxicology</jtitle><stitle>Journal of Analytical Toxicology</stitle><addtitle>Journal of Analytical Toxicology</addtitle><date>1997-01-01</date><risdate>1997</risdate><volume>21</volume><issue>1</issue><spage>44</spage><epage>48</epage><pages>44-48</pages><issn>0146-4760</issn><eissn>1945-2403</eissn><coden>JATOD3</coden><abstract>A sensitive, monoclonal antibody-based ELISA test was developed and used for quantitative determination of ochratoxin A (OA) in human sera. The measuring range of this test (without sample dilution) was 0.2–2.0 ng/mL, and the detection limit was 0.2 ng/mL. The OA concentrations of 355 sera samples varied from <0.2 to 10 ng/mL OA, but 75% of the samples contained 0.2–1.0 ng/mL. This amount reflects a tolerable daily intake (TDI) value of toxin. However, in some cases (6.8%), more than 1.0 ng/mL OA was measured, which is probably a result of elevated intake of OA, which may even exceed the “virtually safe dose”. Our data indicate that, like in many other countries, OA is present in food or feed products available in Hungary, and in order to save the health of consumers, their regular control is desirable.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>9013292</pmid><doi>10.1093/jat/21.1.44</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antibodies, Monoclonal - analysis Biological and medical sciences Consumers Data processing Edible Grain - chemistry Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Food Food toxicology Humans Hungary Medical sciences Mycotoxins - blood Ochratoxin A Ochratoxins - blood Osteoarthritis Reproducibility of Results Sensitivity and Specificity Toxicology Toxins |
title | Ochratoxin A Content of Human Sera Determined by a Sensitive ELISA |
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