Determination of pathways of glycogen synthesis and the dilution of the three-carbon pool with (U- super(13)C)glucose

Rats were infused with glucose at 30 mg/min, containing 18% enriched (U- super(13)C)glucose and (1- super(14)C)- and (3- super(3)H)glucose. The mass isotopomer patterns of super(13)C-labeled blood glucose and liver glycogen were determined by gas chromatography/mass spectroscopy. Methods to calculat...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1991-01, Vol.88 (6), p.2103-2107
Main Authors: Katz, J, Wals, P A, Lee, WNP
Format: Article
Language:English
Subjects:
liver
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Summary:Rats were infused with glucose at 30 mg/min, containing 18% enriched (U- super(13)C)glucose and (1- super(14)C)- and (3- super(3)H)glucose. The mass isotopomer patterns of super(13)C-labeled blood glucose and liver glycogen were determined by gas chromatography/mass spectroscopy. Methods to calculate the two types of dilution are presented.
ISSN:0027-8424