Production and regeneration of protoplasts from the mycorrhizal fungus Suillus granulatus

Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×10(7)/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was M...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1996-11, Vol.12 (6), p.625-628
Main Authors: DIAS, E. S, ARAUJO, E. F, GUIMARAES, W. V, MUCHOVEJ, R. M. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Pinus caribaea hondurensis
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Summary:Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×10(7)/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was MgSO4 (0.7 M). To optimize protoplast release and regeneration an enzyme (Novozym 234) concentration 1.7 mg/ml was chosen, with a digestion time of 1 to 2 h. Regenerated colonies formed mycorrhizae within 60 days after inoculation in Pinus caribaea var. hondurensis seedlings.
ISSN:0959-3993
1573-0972
DOI:10.1007/bf00327726