Dephosphorylation and activation of a p34 super(cdc2)/cyclin B complex in vitro by human CDC25 protein

We have purified the inactive p34 super(cdc2)/cyclin B complex from G2-arrested starfish oocytes. Addition of the purified bacterially expressed product of the human homologue of the fission yeast cdc25 super(+) gene (p54 super(CDC25H)) triggers p34 super(cdc2) dephosphorylation and activates H1 his...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1991-01, Vol.351 (6323), p.242-245
Main Authors: Strausfeld, U, Labbe, J C, Fesquet, D, Cavodore, J C, Picard, A, Sadhu, K, Russell, P, Doree, M
Format: Article
Language:English
Subjects:
CDC25 H protein
cyclin B
protein kinase
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have purified the inactive p34 super(cdc2)/cyclin B complex from G2-arrested starfish oocytes. Addition of the purified bacterially expressed product of the human homologue of the fission yeast cdc25 super(+) gene (p54 super(CDC25H)) triggers p34 super(cdc2) dephosphorylation and activates H1 histone kinase activity in this preparation. We propose that the cdc25 super(+) gene product directly activates the p34 super(cdc2)-cyclin B complex.
ISSN:0028-0836