The effects of the detergent LDAO on the carotenoid metabolism and growth of Rhodovulum sulfidophilum

Carotenoids play an important role in the purple non-sulfur bacteria. In this work we show that addition of the zwitterionic detergent LDAO (lauryldimethylamine N-oxide) to the growth media of Rhodovulum sulfidophilum results in a drastic alteration of the carotenoid biosynthesis, leading to the acc...

Full description

Saved in:
Bibliographic Details
Published in:Microbiological research 1996-12, Vol.151 (4), p.421-426
Main Authors: Hagemann, Gesine E., Sturgis, James N., Wagner, Edgar, Robert, Bruno, Beyer, Peter, Tadros, Monier H.
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacteriology
Biological and medical sciences
carotenoid
detergent
Fundamental and applied biological sciences. Psychology
LDAO
Microbiology
Rhodovulum sulfidophilum
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Carotenoids play an important role in the purple non-sulfur bacteria. In this work we show that addition of the zwitterionic detergent LDAO (lauryldimethylamine N-oxide) to the growth media of Rhodovulum sulfidophilum results in a drastic alteration of the carotenoid biosynthesis, leading to the accumulation of the precursor neurosporene instead of carotenoids of the spheroidene series. This is probably due to a specific inhibition of the hydroxylation of neurosporene either by direct inhibition of the hydroxylase or by disruption of a presumed carotenoid synthesis multienzyme complex coordinated by the crtK encoded protein. Neurosporene can be integrated into peripheral light harvesting complexes (LHII), and is capable of efficient singlet energy transfer, but the resulting LHII complexes show a reduced stability relative to those containing normal carotenoids.
ISSN:0944-5013
1618-0623
DOI:10.1016/S0944-5013(96)80012-7