Functional expression of the rat heart I Na super(+) channel isoform. Demonstration of properties characteristic of native cardiac Na super(+) channels

We describe the expression of functional Na super(+) channels in Xenopus) oocytes injected with cRNA transcribed from the rat heart I cDNA clone. The expressed rat heart I Na super(+) currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native car...

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Bibliographic Details
Published in:FEBS letters 1990-01, Vol.275 (1-2), p.195-200
Main Authors: Cribbs, L L, Satin, J, Fozzard, HA, Rogart, R B
Format: Article
Language:English
Subjects:
oocytes
sodium
Online Access:Get full text
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Summary:We describe the expression of functional Na super(+) channels in Xenopus) oocytes injected with cRNA transcribed from the rat heart I cDNA clone. The expressed rat heart I Na super(+) currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na super(+) currents. The primary amino acid sequence of the rat heart I alpha -subunit is therefore sufficient for expression of tetrodotoxin resistance, and the rat heart I clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.
ISSN:0014-5793