chemo-enzymatic hydrolysis of calcium salt of RNA to nucleosides

Ribonucleosides can be produced by calcium-catalyzed cleavage of RNA followed by dephosphorylation of calcium salts of mononucleotides by intact mycelium of Spicaria violacea as a biocatalyst. Under optimal conditions the efficiency of degradation of baker's yeast RNA to a mixture of ribonucleo...

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Bibliographic Details
Published in:Biotechnology techniques 1996-11, Vol.10 (11), p.875-878
Main Authors: Barai, V.N, Zinchenko, A.I, Bravsevich, I.A, Mikhailopulo, I.A
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
calcium
catalytic activity
enzyme activity
Fundamental and applied biological sciences. Psychology
hydrolysis
inorganic ions
Methods. Procedures. Technologies
phosphoric monoester hydrolases
ribonucleosides
RNA
spicaria
spicaria violacea
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Summary:Ribonucleosides can be produced by calcium-catalyzed cleavage of RNA followed by dephosphorylation of calcium salts of mononucleotides by intact mycelium of Spicaria violacea as a biocatalyst. Under optimal conditions the efficiency of degradation of baker's yeast RNA to a mixture of ribonucleosides is 96% of the theoretical value.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00154676