Detection of PR-b proteins in tobacco roots infected with Chalara elegans

All previous studies on pathogenesis-related (b) protein (PR-b) induction in tobacco have been carried out on leaves or callus tissue. This paper reports the production of PR-b proteins also in roots of tobacco plants (Nicotiana tabacum cv. Xanthi NC) infected with Chalara elegans. Antiserum against...

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Bibliographic Details
Published in:Plant molecular biology 1990-05, Vol.14 (5), p.869-871
Main Authors: TAHIRI-ALAOUI, A, DUMAS, E, GIANINAZZI, S
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Economic plant physiology
Fundamental and applied biological sciences. Psychology
Generalities and techniques
Immunochemistry
Mitosporic Fungi - pathogenicity
Nicotiana - metabolism
Nicotiana - microbiology
Nicotiana tabacum
Plant Proteins - biosynthesis
Plant Proteins - immunology
Plants - metabolism
Plants - microbiology
Plants, Toxic
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Summary:All previous studies on pathogenesis-related (b) protein (PR-b) induction in tobacco have been carried out on leaves or callus tissue. This paper reports the production of PR-b proteins also in roots of tobacco plants (Nicotiana tabacum cv. Xanthi NC) infected with Chalara elegans. Antiserum against PR-b1 reacted with PR-b1, PR-b2 and PR-b3 and gave the same pattern of reaction as for leaves. Antiserum against PR-b5 revealed the presence of PR-b4, PR-b5 and, very weakly, PR-b6 which have been shown to be beta-1,3 glucanases. Antiserum against PR-b7 reacted with both PR-b7 and PR-b8 which are chitinases.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00016522