Evaluation of a visual, rapid, membrane enzyme immunoassay for the detection of herpes simples virus antigen

We evaluated a 12-min, direct, monoclonal antibody-based enzyme immunoassay (EIA) (SureCell; Kodak, Rochester, N.Y.) which aids in the detection of herpes simplex virus infection; the assay system is also approved for culture confirmation. The test was evaluated from direct clinical samples and comp...

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Published in:Journal of clinical microbiology 1991-01, Vol.29 (4), p.842-845
Main Authors: Zimmerman, S J, Moses, E, Sofat, N, Bartholomew, W R, Amsterdam, D
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container_title Journal of clinical microbiology
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creator Zimmerman, S J
Moses, E
Sofat, N
Bartholomew, W R
Amsterdam, D
description We evaluated a 12-min, direct, monoclonal antibody-based enzyme immunoassay (EIA) (SureCell; Kodak, Rochester, N.Y.) which aids in the detection of herpes simplex virus infection; the assay system is also approved for culture confirmation. The test was evaluated from direct clinical samples and compared with conventional culture methodology by using a single swab. A total of 265 specimens from 180 female cervical-urogenital sites, 62 male urogenital sites, 4 rectal sites, 3 skin sites, 6 oral sites, and 10 colposcopy sites were collected on Dacron or cotton swabs and placed in viral transport medium. Variables affecting the EIA sensitivity are the stage of the lesion and conventional culture methodologies. A review of culture results for 32 EIA false-negative tests indicated that 15 were detected after 48 h of incubation. Cytopathic effect observed at 48-, 72-, and 96-h cutoffs altered the sensitivity for the EIA. To ensure detection of SureCell herpes simplex virus-negative specimens, it is recommended that n unused aliquot of VTM be tested in cell culture.
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title Evaluation of a visual, rapid, membrane enzyme immunoassay for the detection of herpes simples virus antigen
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