In vitro activity of omeprazole in combination with several antimicrobial agents against clinical isolates of Helicobacter pylori

An agar dilution checkerboard method was used to evaluate the in vitro activity of omeprazole combined with clarithromycin, amoxicillin, and ceftibuten, respectively, against clinical isolates of Helicobacter pylori. Mueller-Hinton agar plus 5% horse blood, an inoculum of 10(6) cfu/ml, and incubatio...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1996-12, Vol.15 (12), p.937-940
Main Authors: ALARCON, T, DOMINGO, D, SANCHEZ, I, DIAZ DE ROJAS, F, LOPEZ-BREA, M
Format: Article
Language:English
Subjects:
Amoxicillin - pharmacology
Anti-Bacterial Agents - pharmacology
Anti-Ulcer Agents - pharmacology
Biological and medical sciences
Biopsy
Ceftibuten
Cephalosporins - pharmacology
Clarithromycin - pharmacology
Digestive system
Drug Antagonism
Drug Synergism
Drug Therapy, Combination
Endoscopy
Helicobacter Infections - drug therapy
Helicobacter pylori
Helicobacter pylori - drug effects
Humans
Medical sciences
Microbial Sensitivity Tests
Omeprazole - pharmacology
Penicillins - pharmacology
Pharmacology. Drug treatments
Stomach - microbiology
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Summary:An agar dilution checkerboard method was used to evaluate the in vitro activity of omeprazole combined with clarithromycin, amoxicillin, and ceftibuten, respectively, against clinical isolates of Helicobacter pylori. Mueller-Hinton agar plus 5% horse blood, an inoculum of 10(6) cfu/ml, and incubation of 72 h in a CO2 atmosphere were used. With the omeprazole and clarithromycin combination, synergism was observed in 51.5% and partial synergism in 39.3% of 33 strains; with omeprazole and amoxicillin, synergism was observed in 3% and partial synergism in 60.6% of 33 strains; and with omeprazole and ceftibuten, synergism was observed in 33.3% and partial synergism in 50% of 24 strains. Antagonism between omeprazole and each antibiotic was exhibited in 0%, 6.1%, and 4.1% of these groups of strains, respectively. Of the antibiotic combinations tested, omeprazole plus clarithromycin exhibited synergism (partial or total) in the highest percentage of strains.
ISSN:0934-9723
1435-4373
DOI:10.1007/BF01690512