Retron for the 67-Base Multicopy Single-Stranded DNA from Escherichia coli: A Potential Transposable Element Encoding Both Reverse Transcriptase and Dam Methylase Functions

The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilo...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1990-12, Vol.87 (23), p.9454-9458
Main Authors: Hsu, Mei-Yin, Inouye, Masayori, Inouye, Sumiko
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Bacteriophages
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosomes
Chromosomes, Bacterial
Dams
DNA
DNA Transposable Elements
DNA, Single-Stranded - genetics
Escherichia coli - genetics
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Genes
Genic rearrangement. Recombination. Transposable element
Genomes
Methyltransferases - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleotide sequences
Open reading frames
Restriction Mapping
RNA-Directed DNA Polymerase - genetics
Sequence Homology, Nucleic Acid
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Transposons
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Summary:The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position equivalent to 19 min on the K-12 chromosome. The element containing the retron consisted of a unique 34-kilobase sequence that was flanked by direct repeats of a 26-base-pair sequence found in the K-12 chromosomal DNA. This suggests that the 34-kilobase element was probably integrated into the E. coli genome by a mechanism related to transposition or phage integration. In the 34-kilobase sequence an open reading frame of 285 residues was found, which displays 44% sequence identity with the E. coli Dam methylase. Interestingly, there are three GATC sequences, the site of Dam methylation, in the promoter region of the gene for reverse transcriptase.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.87.23.9454