Distribution of the insertion element IS240 among Bacillus thuringiensis strains

The presence of IS240 was investigated in 69 Bacillus thuringiensis (Bt) strains including strains from serotype H1 to H45 and additional strains with known Dipteran larvae toxicity. Restriction digests of total DNA and PCR products obtained with a single 16-bases primer corresponding to the IS240 i...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 1997-06, Vol.34 (6), p.348-353
Main Authors: Rosso, M.L. (Institut Pasteur, Paris, France.), Delecluse, A
Format: Article
Language:English
Subjects:
Animals
BACILLUS THURINGIENSIS
Bacillus thuringiensis - classification
Bacillus thuringiensis - genetics
Bacillus thuringiensis - pathogenicity
Bacteriology
Biological and medical sciences
DIPTERA
Diptera - microbiology
DNA Transposable Elements
Fundamental and applied biological sciences. Psychology
Genetics
Larvae
Microbiology
Mosquito Control
Pest Control, Biological
Serotyping
Species Specificity
TOXICIDAD
TOXICITE
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The presence of IS240 was investigated in 69 Bacillus thuringiensis (Bt) strains including strains from serotype H1 to H45 and additional strains with known Dipteran larvae toxicity. Restriction digests of total DNA and PCR products obtained with a single 16-bases primer corresponding to the IS240 inverted repeated sequence were hybridized with the IS240A element. The results indicate that 67% of the Bt strains tested, including all known mosquitocidal strains, possess at least one IS240-related element. PCR experiments indicate that IS240 represents a family of insertion sequences with several variants
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900194