Malignant transformation of a papilloma induced by bovine papillomavirus type 4 in the nude mouse renal capsule

A papillomatous cyst was induced by implanting bovine foetal palate epithelium, infected in vitro with bovine papillomavirus type 4 (BPV-4), beneath the renal capsule of a nude mouse. The benign tumour underwent malignant progression, developing into a squamous cell carcinoma with metastatic deposit...

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Bibliographic Details
Published in:Journal of general virology 1991-05, Vol.72 (5), p.1165-1168
Main Authors: Gaukroger, J, Chandrachud, L, Jarrett, W.F.H, McGarvie, G.E, Yeudall, W.A, McCaffery, R.E, Smith, K.T, Campo, M.S
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - analysis
Base Sequence
Biological and medical sciences
Bovine papillomavirus
Cattle
Cell Transformation, Neoplastic
Cell Transformation, Viral
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Kidney Neoplasms - chemistry
Kidney Neoplasms - microbiology
Kidney Neoplasms - pathology
kidneys
Major Histocompatibility Complex - immunology
Mice
Mice, Nude
Microbiology
Molecular Sequence Data
papilloma
Papilloma - chemistry
Papilloma - microbiology
Papilloma - pathology
Papillomaviridae - isolation & purification
Papillomaviridae - physiology
Polymerase Chain Reaction
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Splenic Neoplasms - secondary
Virology
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Summary:A papillomatous cyst was induced by implanting bovine foetal palate epithelium, infected in vitro with bovine papillomavirus type 4 (BPV-4), beneath the renal capsule of a nude mouse. The benign tumour underwent malignant progression, developing into a squamous cell carcinoma with metastatic deposits in the spleen. The bovine origin of both the renal and splenic cancers was confirmed by the presence of bovine major histocompatibility complex class I antigens in the cancer cells and by sequencing the Harvey-ras1 gene, which was shown to be of bovine origin. BPV-4 DNA was present in the residual papillomatous fronds of the renal cancer, but was absent from the carcinoma proper and for the splenic metastasis. These results confirm that BPV-4 is a carcinogenic agent and that its genetic information is not necessary for the maintenance of the malignant phenotype. Moreover the system provides the opportunity to investigate the role of viral and chemical carcinogens in an experimental system.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-72-5-1165