Increase of xanthan production by self-cloning of a 3.0-kb EcoRI-KpnI chromosomal fragment in Xanthomonas campestris

G76E is a non-mucoid mutant derived from Xanthomonas campestris pv. campestris 17 by Tn mutagenesis. A 3.0-kb EcoRI-KpnI fragment, cloned from a genomic bank of Xc17, was able to complement G76E and caused an increase of xanthan production by ca. 46% over Xc17. This 3.0-kb EcoBI-KpnI fragment insert...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 1996-11, Vol.18 (11), p.1301-1304
Main Authors: Wei, C.L, Choy, K.T, Lin, N.T, Tseng, Y.H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular cloning
Xanthomonas campestris
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:G76E is a non-mucoid mutant derived from Xanthomonas campestris pv. campestris 17 by Tn mutagenesis. A 3.0-kb EcoRI-KpnI fragment, cloned from a genomic bank of Xc17, was able to complement G76E and caused an increase of xanthan production by ca. 46% over Xc17. This 3.0-kb EcoBI-KpnI fragment insert was different from the two previously cloned EcoRI fragments from Xc17 that also increased xanthan production.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00129959