Development of a nontoxic acoustic window nutrient-mist bioreactor and relevant growth data

A nutrient-mist bioreactor was designed that separates the nutrient medium from the electronic components via an acoustic window. This eliminates compromising culture sterility when repairing mechanical failures common with commercially available mist reactors. The experimental mist bioreactor is lo...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1996-10, Vol.32 (4), p.299-304
Main Authors: Buer, C.S. (Worcester Polytechnic Institute, Worcester, MA.), Correll, M.J, Smith, T.C, Towler, M.J, Weathers, P.J, Nadler, M, Seaman, J, Walcerz, D
Format: Article
Language:English
Subjects:
Acoustical materials
Air flow
ARTEMISIA
ARTEMISIA ANNUA
Biological and medical sciences
BIOREACTEUR
BIOREACTORS
BIORREACTORES
CULTIVO DE TEJIDOS
CULTURE DE TISSU
CULTURE MEDIA
EQUIPO
Flasks
Fundamental and applied biological sciences. Psychology
General aspects, methods
HAIRY ROOTS
Liquids
MATERIEL
MEDIO DE CULTIVO
MILIEU DE CULTURE
MIST APPLICATION
MIST SPRAYERS
NEPHROLEPIS
NEPHROLEPIS EXALTATA
NUTRIENT SOLUTIONS
Phytotrons
Plant physiology and development
Plant roots
PULVERISATEUR
PULVERISATION
PULVERIZACION
PULVERIZADORES
RACINE
RAICES
ROOTS
Secondary Metabolism
SOLUCIONES NUTRITIVAS
SOLUTION NUTRITIVE
SPRAYERS
SPRAYING
STEMS
TALLO
TIGE
TISSUE CULTURE
Toxicity tests
TRANSDUCERS
ULTRASON
ULTRASONIC TRANSDUCERS
ULTRASONICS
ULTRASONIDO
Weather
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Description
Summary:A nutrient-mist bioreactor was designed that separates the nutrient medium from the electronic components via an acoustic window. This eliminates compromising culture sterility when repairing mechanical failures common with commercially available mist reactors. The experimental mist bioreactor is low cost and can be assembled in any laboratory. Toxicity tests of several potential acoustically transparent materials are included. Details of the construction procedures include methods for casting the window. Growth data using the newly designed nutrient mist bioreactor are compared to data from a commercial mist reactor, shake flasks, and Gelrite cultures. Artemisia annua hairy roots and Nephrolepis exaltata shoot cultures showed growth comparable to the conventional tissue culture methods.
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02822703