Diabetes-induced alterations in the expression, functioning and phosphorylation state of the inhibitory guanine nucleotide regulatory protein G sub(i)-2 in hepatocytes

Levels of the G-protein alpha -subunits alpha -G sub(i)-2, alpha -G sub(i)-3 and the 42 kDa, but not the 45 kDa, form of alpha -G sub(s) were markedly decreased in hepatocyte membranes from streptozotocin-diabetic animals as compared with normals. In contrast, no detectable changes in alpha -G sub(i...

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Bibliographic Details
Published in:Biochemical journal 1990-01, Vol.271 (2), p.365-372
Main Authors: Bushfield, M, Griffiths, S L, Murphy, G J, Pyne, N J, Knowler, J T, Milligan, G, Parker, P J, Mollner, S, Houslay, MD
Format: Article
Language:English
Subjects:
diabetes mellitus
guanine nucleotide-binding protein
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Summary:Levels of the G-protein alpha -subunits alpha -G sub(i)-2, alpha -G sub(i)-3 and the 42 kDa, but not the 45 kDa, form of alpha -G sub(s) were markedly decreased in hepatocyte membranes from streptozotocin-diabetic animals as compared with normals. In contrast, no detectable changes in alpha -G sub(i) subunits were seen in liver plasma membranes of streptozotocin-diabetic animals, although levels of the 45 kDa form of G sub(s) were increased. G-protein beta subunits in plasma membranes were unaffected by diabetes induction. Analysis of whole-liver RNA indicated that the induction of diabetes had little effect on transcript levels of G sub(i)-3, caused an increase in G sub(s) transcripts and decreased transcript number for G sub(i)-2, albeit to a much lesser extent than was observed upon analysis of hepatocyte RNA.
ISSN:0264-6021