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ω-Agatoxin IVA blocks spinal morphine/clonidine antinociceptive synergism
Involvement of P-type voltage-dependent Ca 2+ channels in spinal morphine- or clonidine-induced antinociception and in the synergistic interaction between morphine and clonidine was examined in the present studies. Coadministration of the selective P-type antagonist, ω-agatoxin IVA (25 ng) intrathec...
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| Published in: | European journal of pharmacology 1996-10, Vol.314 (3), p.293-300 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c420t-a985053b396eed63160b87d36fa912a61f2805fa2f1d35de67ee6ee720533c9c3 |
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| cites | cdi_FETCH-LOGICAL-c420t-a985053b396eed63160b87d36fa912a61f2805fa2f1d35de67ee6ee720533c9c3 |
| container_end_page | 300 |
| container_issue | 3 |
| container_start_page | 293 |
| container_title | European journal of pharmacology |
| container_volume | 314 |
| creator | Roerig, Sandra C. Howse, Kurt M. |
| description | Involvement of P-type voltage-dependent Ca
2+ channels in spinal morphine- or clonidine-induced antinociception and in the synergistic interaction between morphine and clonidine was examined in the present studies. Coadministration of the selective P-type antagonist, ω-agatoxin IVA (25 ng) intrathecally (i.t.) to mice along with morphine or clonidine enhanced the tail flick antinociception of each agonist 5–6-fold. The greater-than-additive (synergistic) interaction that occurred when morphine and clonidine were coadministered i.t. decreased to an additive interaction in the presence of ω-agatoxin IVA. In mice pretreated with pertussis toxin (10 ng) to inactivate G proteins, ω-agatoxin IVA did not alter the morphine/clonidine synergism. Surprisingly, ω-agatoxin IVA reversed the additive morphine/clonidine interaction that occurs in morphine-tolerant mice back to synergism. These results suggest that functional P-type Ca
2+ channels play an essential role in the antinociceptive synergism between spinal morphine and clonidine. |
| doi_str_mv | 10.1016/S0014-2999(96)00561-4 |
| format | article |
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2+ channels in spinal morphine- or clonidine-induced antinociception and in the synergistic interaction between morphine and clonidine was examined in the present studies. Coadministration of the selective P-type antagonist, ω-agatoxin IVA (25 ng) intrathecally (i.t.) to mice along with morphine or clonidine enhanced the tail flick antinociception of each agonist 5–6-fold. The greater-than-additive (synergistic) interaction that occurred when morphine and clonidine were coadministered i.t. decreased to an additive interaction in the presence of ω-agatoxin IVA. In mice pretreated with pertussis toxin (10 ng) to inactivate G proteins, ω-agatoxin IVA did not alter the morphine/clonidine synergism. Surprisingly, ω-agatoxin IVA reversed the additive morphine/clonidine interaction that occurs in morphine-tolerant mice back to synergism. These results suggest that functional P-type Ca
2+ channels play an essential role in the antinociceptive synergism between spinal morphine and clonidine.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/S0014-2999(96)00561-4</identifier><identifier>PMID: 8957249</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analgesics, Opioid - antagonists & inhibitors ; Animals ; Antinociception, spinal ; Biological and medical sciences ; Ca 2+ channel, P-type ; Calcium Channel Blockers - pharmacology ; Clonidine ; Clonidine - antagonists & inhibitors ; Drug Synergism ; Drug Tolerance ; G-protein ; General pharmacology ; Linear Models ; Male ; Medical sciences ; Mice ; Mice, Inbred ICR ; Morphine ; Morphine - antagonists & inhibitors ; Morphine Dependence - prevention & control ; omega-Agatoxin IVA ; Pertussis Toxin ; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions ; Pharmacology. Drug treatments ; Spider Venoms - pharmacology ; Spinal Cord - drug effects ; Substance-Related Disorders - prevention & control ; Tolerance ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>European journal of pharmacology, 1996-10, Vol.314 (3), p.293-300</ispartof><rights>1996</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a985053b396eed63160b87d36fa912a61f2805fa2f1d35de67ee6ee720533c9c3</citedby><cites>FETCH-LOGICAL-c420t-a985053b396eed63160b87d36fa912a61f2805fa2f1d35de67ee6ee720533c9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2506602$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8957249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roerig, Sandra C.</creatorcontrib><creatorcontrib>Howse, Kurt M.</creatorcontrib><title>ω-Agatoxin IVA blocks spinal morphine/clonidine antinociceptive synergism</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Involvement of P-type voltage-dependent Ca
2+ channels in spinal morphine- or clonidine-induced antinociception and in the synergistic interaction between morphine and clonidine was examined in the present studies. Coadministration of the selective P-type antagonist, ω-agatoxin IVA (25 ng) intrathecally (i.t.) to mice along with morphine or clonidine enhanced the tail flick antinociception of each agonist 5–6-fold. The greater-than-additive (synergistic) interaction that occurred when morphine and clonidine were coadministered i.t. decreased to an additive interaction in the presence of ω-agatoxin IVA. In mice pretreated with pertussis toxin (10 ng) to inactivate G proteins, ω-agatoxin IVA did not alter the morphine/clonidine synergism. Surprisingly, ω-agatoxin IVA reversed the additive morphine/clonidine interaction that occurs in morphine-tolerant mice back to synergism. These results suggest that functional P-type Ca
2+ channels play an essential role in the antinociceptive synergism between spinal morphine and clonidine.</description><subject>Analgesics, Opioid - antagonists & inhibitors</subject><subject>Animals</subject><subject>Antinociception, spinal</subject><subject>Biological and medical sciences</subject><subject>Ca 2+ channel, P-type</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Clonidine</subject><subject>Clonidine - antagonists & inhibitors</subject><subject>Drug Synergism</subject><subject>Drug Tolerance</subject><subject>G-protein</subject><subject>General pharmacology</subject><subject>Linear Models</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Morphine</subject><subject>Morphine - antagonists & inhibitors</subject><subject>Morphine Dependence - prevention & control</subject><subject>omega-Agatoxin IVA</subject><subject>Pertussis Toxin</subject><subject>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</subject><subject>Pharmacology. Drug treatments</subject><subject>Spider Venoms - pharmacology</subject><subject>Spinal Cord - drug effects</subject><subject>Substance-Related Disorders - prevention & control</subject><subject>Tolerance</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkMlOwzAQhi0EgrI8QqUcEIJDwHZiJz6hqmIVEgeWq-U6EzAkdrDTij4CT8cr4S7qldOMNN8_M_oQGhJ8TjDhF08YkzylQohTwc8wZpyk-RYakLIQKS4I3UaDDbKH9kP4wJESlO2i3VKwguZigO5_f9LRm-rdt7HJ3esomTROf4YkdMaqJmmd796NhQvdOGuq2CXK9sY6bTR0vZlBEuYW_JsJ7SHaqVUT4GhdD9DL9dXz-DZ9eLy5G48eUp1T3KdKlAyzbJIJDlDxjHA8KYsq47UShCpOalpiVitakypjFfACIJIFjaFMC50doJPV3s67rymEXrYmaGgaZcFNg4wLM8pzGkG2ArV3IXioZedNq_xcEiwXDuXSoVwIkoLLpUOZx9xwfWA6aaHapNbS4vx4PVdBq6b2ymoTNhhlmHO8OH-5wiDKmBnwMmgDVkNlPOheVs7888gfSbCOQw</recordid><startdate>19961031</startdate><enddate>19961031</enddate><creator>Roerig, Sandra C.</creator><creator>Howse, Kurt M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope></search><sort><creationdate>19961031</creationdate><title>ω-Agatoxin IVA blocks spinal morphine/clonidine antinociceptive synergism</title><author>Roerig, Sandra C. ; Howse, Kurt M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-a985053b396eed63160b87d36fa912a61f2805fa2f1d35de67ee6ee720533c9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analgesics, Opioid - antagonists & inhibitors</topic><topic>Animals</topic><topic>Antinociception, spinal</topic><topic>Biological and medical sciences</topic><topic>Ca 2+ channel, P-type</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Clonidine</topic><topic>Clonidine - antagonists & inhibitors</topic><topic>Drug Synergism</topic><topic>Drug Tolerance</topic><topic>G-protein</topic><topic>General pharmacology</topic><topic>Linear Models</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Morphine</topic><topic>Morphine - antagonists & inhibitors</topic><topic>Morphine Dependence - prevention & control</topic><topic>omega-Agatoxin IVA</topic><topic>Pertussis Toxin</topic><topic>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</topic><topic>Pharmacology. Drug treatments</topic><topic>Spider Venoms - pharmacology</topic><topic>Spinal Cord - drug effects</topic><topic>Substance-Related Disorders - prevention & control</topic><topic>Tolerance</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roerig, Sandra C.</creatorcontrib><creatorcontrib>Howse, Kurt M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roerig, Sandra C.</au><au>Howse, Kurt M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ω-Agatoxin IVA blocks spinal morphine/clonidine antinociceptive synergism</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1996-10-31</date><risdate>1996</risdate><volume>314</volume><issue>3</issue><spage>293</spage><epage>300</epage><pages>293-300</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Involvement of P-type voltage-dependent Ca
2+ channels in spinal morphine- or clonidine-induced antinociception and in the synergistic interaction between morphine and clonidine was examined in the present studies. Coadministration of the selective P-type antagonist, ω-agatoxin IVA (25 ng) intrathecally (i.t.) to mice along with morphine or clonidine enhanced the tail flick antinociception of each agonist 5–6-fold. The greater-than-additive (synergistic) interaction that occurred when morphine and clonidine were coadministered i.t. decreased to an additive interaction in the presence of ω-agatoxin IVA. In mice pretreated with pertussis toxin (10 ng) to inactivate G proteins, ω-agatoxin IVA did not alter the morphine/clonidine synergism. Surprisingly, ω-agatoxin IVA reversed the additive morphine/clonidine interaction that occurs in morphine-tolerant mice back to synergism. These results suggest that functional P-type Ca
2+ channels play an essential role in the antinociceptive synergism between spinal morphine and clonidine.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8957249</pmid><doi>10.1016/S0014-2999(96)00561-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 1996-10, Vol.314 (3), p.293-300 |
| issn | 0014-2999 1879-0712 |
| language | eng |
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| source | Elsevier |
| subjects | Analgesics, Opioid - antagonists & inhibitors Animals Antinociception, spinal Biological and medical sciences Ca 2+ channel, P-type Calcium Channel Blockers - pharmacology Clonidine Clonidine - antagonists & inhibitors Drug Synergism Drug Tolerance G-protein General pharmacology Linear Models Male Medical sciences Mice Mice, Inbred ICR Morphine Morphine - antagonists & inhibitors Morphine Dependence - prevention & control omega-Agatoxin IVA Pertussis Toxin Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions Pharmacology. Drug treatments Spider Venoms - pharmacology Spinal Cord - drug effects Substance-Related Disorders - prevention & control Tolerance Virulence Factors, Bordetella - pharmacology |
| title | ω-Agatoxin IVA blocks spinal morphine/clonidine antinociceptive synergism |
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