The effects of amiloride on electrolyte transport and acid-base balance in the larval salamander, Ambystoma tigrinum

The responses of net and unidirectional fluxes of Na super(+) and acid-base balance to the drug amiloride were assessed during normocapnia and hypercapnia in larval salamanders, Ambystoma tigrinum). Isotope flux measurements demonstrated that 10 super(-4) M amiloride in the external medium inhibits...

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Bibliographic Details
Published in:Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Biochemical, systemic, and environmental physiology, 1991-09, Vol.161 (5), p.460-464
Main Authors: CROCKER, C. E, STIFFLER, D. F
Format: Article
Language:English
Subjects:
Ambystoma tigrinum
Biological and medical sciences
biological membranes
Blood gas. Hemoglobin. Myoglobin. Hemotissulary gas exchange. Acid-base balance
effects on
electrolytes
Freshwater
Fundamental and applied biological sciences. Psychology
hypercapnia
ion transport
pharmacology
protons
sodium
Vertebrates: respiratory system
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Summary:The responses of net and unidirectional fluxes of Na super(+) and acid-base balance to the drug amiloride were assessed during normocapnia and hypercapnia in larval salamanders, Ambystoma tigrinum). Isotope flux measurements demonstrated that 10 super(-4) M amiloride in the external medium inhibits Na super(+) influx during normocapnia and reverses the usual increase in influx of this ion during hypercapnia, causing a significant decrease instead. Measurements of blood-gas/acid-base balance conditions of arterially cannulated salamanders demonstrated a significant metabolic acidosis in amiloride-treated animals that did not occur in untreated animals over the same period. The same concentration of amiloride also blocked the normal compensatory increase in (HCO super(d)3 super(-)) that follows a respiratory acidosis produced by a hypercapnic environment.
ISSN:0174-1578
1432-136X
DOI:10.1007/BF00257900