Immunoassay identification of Aspergillus flavus using monoclonal antibodies raised to the whole cell extracts

Five separate monoclonal antibodies were produced against whole cell extracts of Aspergillus flavus and ELISA procedures used to characterise the reactivity of the antibodies to various fungal extracts. All five antibodies were specific to the aflatoxin producing fungi, A. flavus and A. parasiticus,...

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Bibliographic Details
Published in:Biotechnology techniques 1997, Vol.11 (1), p.21-24
Main Authors: CANDLISH, A. A. G, WIBOWO, M. S, SMITH, J. E
Format: Article
Language:English
Subjects:
Aspergillus flavus
Aspergillus parasiticus
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Isolation and description
Microbiology
Mission oriented research
Mycological methods and techniques used in mycology
Mycology
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Summary:Five separate monoclonal antibodies were produced against whole cell extracts of Aspergillus flavus and ELISA procedures used to characterise the reactivity of the antibodies to various fungal extracts. All five antibodies were specific to the aflatoxin producing fungi, A. flavus and A. parasiticus, and indicated no cross reactivity with other Aspergillus species, genera of several fungi or with other components which may be found in food samples where A. flavus may be found.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF02764445