Distribution of endosomal, lysosomal, and major histocompatibility complex markers in a monocytic cell line infected with Chlamydia psittaci

The intracellular fate of Chlamydia psittaci during infection of a monocytic cell line, THP1, was characterized. Cytochalasin D inhibited phagocytosis of latex beads but had no effect on infection by C. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocy...

Full description

Saved in:
Bibliographic Details
Published in:Infection and immunity 1997-06, Vol.65 (6), p.2437-2442
Main Authors: Ojcius, D.M, Hellio, R, Dautry-Varsat, A
Format: Article
Language:English
Subjects:
animal diseases
animal health
bacterial infections
Bacteriology
Biological and medical sciences
Chlamydia psittaci
Fundamental and applied biological sciences. Psychology
human health and safety
medicine
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The intracellular fate of Chlamydia psittaci during infection of a monocytic cell line, THP1, was characterized. Cytochalasin D inhibited phagocytosis of latex beads but had no effect on infection by C. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. Early Chlamydia-containing vacuoles expressed major histocompatibility complex (MHC) class I molecules, and most vacuoles fused with host cell lysosomes, since they expressed LAMP-1 and had acidic pHs. In cells prestimulated with gamma interferon, vacuoles also expressed MHC class II molecules, suggesting that the monocytes might effectively process Chlamydia-derived antigens for presentation by MHC class I and class II molecules.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.65.6.2437-2442.1997