Induction of feline immunodeficiency virus specific antibodies in cats with an attenuated Salmonella strain expressing the Gag protein

Salmonella typhimurium aroA strains (SL3261), expressing high levels of the Gag protein of feline immunodeficiency virus (FIV) fused with maltose binding protein (SL3261-MFG), were constructed using an invertible promoter system that allows the stable expression of heterologous antigens at levels to...

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Bibliographic Details
Published in:Vaccine 1997-04, Vol.15 (6), p.587-596
Main Authors: Tijhaar, Edwin J., Siebelink, Kees H.J., Karlas, Jos A., Burger, Marina C., Mooi, Frits R., Osterhaus, Albert D.M.E.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - biosynthesis
Antibodies, Viral - immunology
Biological and medical sciences
Cats
Chromosome Inversion
feline immunodeficiency virus
FIV
Fundamental and applied biological sciences. Psychology
Gene Expression
Gene Products, gag - genetics
Gene Products, gag - immunology
Genetic Vectors
Immunodeficiency Virus, Feline - immunology
invertible promoter
Microbiology
Plasmids
Salmonella
Salmonella typhimurium
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Attenuated - immunology
Vaccines, Synthetic - immunology
Viral Vaccines - immunology
Virology
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Summary:Salmonella typhimurium aroA strains (SL3261), expressing high levels of the Gag protein of feline immunodeficiency virus (FIV) fused with maltose binding protein (SL3261-MFG), were constructed using an invertible promoter system that allows the stable expression of heterologous antigens at levels toxic for bacteria. A SL3261 strain expressing the B subunit of cholera toxin by a similar system (SL3261-CtxB) served as a control in FIV-immunization experiments. Cats immunized once orally or intraperitoneally with SL3261-MFG or SL3261-CtxB all developed serum antibodies to SL3261 lipopolysaccharide and against maltose binding protein or the B subunit of cholera toxin, respectively. Two intraperitoneal immunizations with SL3261-MFG also resulted in the development of Gag specific serum antibodies. Two oral immunizations with SL3261-MFG primed for a Gag specific response, which was demonstrated upon FIV challenge. All challenged cats became infected and no significant differences in viral loads were found between SL3261-MFG and SL3261-CtxB immunized cats.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(96)00308-8