Determination of imazamethabenz in cereal grain by enzyme-linked immunosorbent assay

Imazamethabenz-methyl (IM) is used in the US and Canada as a postemergent herbicide for wheat and barley cultivation. Plants can hydrolyze IM into free acids. An enzyme-linked immunosorbent assay technique to assess levels of these free acids was developed and tested. Adequate recoveries of imazamet...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of environmental contamination and toxicology 1991-08, Vol.47 (2), p.211-216
Main Authors: Newsome, W.H. (Health and Welfare Canada, Ottawa, Canada), Collins, P.G
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Animals
Biological and medical sciences
Biometrics, statistics, experimental designs, modeling, agricultural computer applications
Edible Grain - analysis
ELISA
Enzyme-Linked Immunosorbent Assay
EXTRACTION
EXTRACTOS VEGETALES
EXTRAIT D'ORIGINE VEGETALE
Fundamental and applied biological sciences. Psychology
Generalities. Biometrics, experimentation. Remote sensing
GRAIN
GRANOS
HERBICIDAS
HERBICIDE
HERBICIDES
Herbicides - analysis
HIDROLISIS
Hordeum - analysis
HORDEUM VULGARE
HYDROLYSE
HYDROLYSIS
METABOLITE
METABOLITES
METABOLITOS
PLANT EXTRACTS
Rabbits - immunology
TEST ELISA
Triticum - analysis
TRITICUM AESTIVUM
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Imazamethabenz-methyl (IM) is used in the US and Canada as a postemergent herbicide for wheat and barley cultivation. Plants can hydrolyze IM into free acids. An enzyme-linked immunosorbent assay technique to assess levels of these free acids was developed and tested. Adequate recoveries of imazamethabenz were achieved with both wheat and barley samples fortified with 12.5-200 ppb IM. Additions of alkali to the extract and a hydrolysis period of 90 min was necessary to ensure conversion of any imazamethabenz when carried through the procedure.
ISSN:0007-4861
1432-0800
DOI:10.1007/BF01688642