Mutational analysis of enhancer domains responsive to trans-activation by the X gene of human hepatitis B virus

The X gene product of human hepatitis B virus (HBV) trans-activates the HBV enhancer. In order to identify domains responsive to trans-activation by the X gene, we introduced a series of mutations into the HBV enhancer and assayed the enhancer activities in the presence of the X gene product. Our re...

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Bibliographic Details
Published in:Archives of virology 1990, Vol.114 (3-4), p.237-242
Main Authors: GOODARZI, G, OHNO, H, ADAMS, R, DARABI, A, TEWARI, A, WATABE, M, WATABE, K
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Chloramphenicol O-Acetyltransferase - genetics
Cloning, Molecular
DNA, Viral
Enhancer Elements, Genetic
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Viral
Genes, Viral
Genetics
Hepatitis B virus - genetics
Humans
Microbiology
Molecular Sequence Data
Mutagenesis, Site-Directed
Open Reading Frames
Trans-Activators - physiology
Tumor Cells, Cultured
Virology
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Summary:The X gene product of human hepatitis B virus (HBV) trans-activates the HBV enhancer. In order to identify domains responsive to trans-activation by the X gene, we introduced a series of mutations into the HBV enhancer and assayed the enhancer activities in the presence of the X gene product. Our results suggest that the EP domain of the enhancer is essential for trans-activation by the X gene.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01310752