A rapid and efficient new assay for determination of the three biotypes of Agrobacterium tumefaciens

We describe a new method for determining the biotype of Agrobacterium tumefaciens strains, based on differential motility in a medium adjusted to different pH levels. Motility of biotype I increases with increasing pH. That of biotype II slightly decreases, but the cells remain motile; the motility...

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Bibliographic Details
Published in:Letters in applied microbiology 1991-09, Vol.13 (3), p.126-129
Main Authors: Bush, A.L., Pueppke, S.G.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
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Summary:We describe a new method for determining the biotype of Agrobacterium tumefaciens strains, based on differential motility in a medium adjusted to different pH levels. Motility of biotype I increases with increasing pH. That of biotype II slightly decreases, but the cells remain motile; the motility of biotype III decreases and the cells become non‐motile. The assay is able to distinguish between strains of all three biotypes within 2 d, and can be applied to relatively large numbers of isolates. Thus it is more rapid and efficient than the frequently used biochemical biotype tests.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1991.tb00588.x