Plant regeneration from protoplasts isolated from primary calli using mature embryos of two Brazilian rice cultivars

• Published in: Euphytica 1997-01, Vol.94 (1), p.1-5
• Main Authors: Moura, D.S. (Sao Paulo Univ. (Brazil). Escola Superior de Agricultura 'Luiz de Queiroz', Dept. de Genetica), Zapata-Arias, F.J, Ando, A, Tulmann Neto, A
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; CAL; CALLO; CALLUS; Cultivars; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CULTURE MEDIA; EMBRIONES VEGETALES; EMBRYON VEGETAL; Embryos; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Haploidy, in vitro culture applications, somatic hybrids; MEDIO DE CULTIVO; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; ORYZA SATIVA; Plant breeding: fundamental aspects and methodology; PLANT EMBRYOS; PROLINA; PROLINE; Rice; TISSUE CULTURE; VARIEDADES; VARIETE; VARIETIES
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1002962724080

A plant regeneration system from rice protoplasts using calli derived from mature embryos was established for the two Brazilian modern rice cultivars IAC-201 and IAC-165. After 30 to 40 days of in vitro culture it was possible to obtain on average 6 million protoplasts per gram of callus. Microscopic selection of embryogenic calli was a key step for protoplast isolation. The production of embryogenic calli increased when L-proline and casein hydrolysate were used in the callus induction medium. The Oc or IR52 nurse cell lines were essential for protoplast division. Different regeneration media were studied and 139 plants were regenerated which set seed. Some of the regenerated plants showed morphological variation such as the presence of awns in spite of the short time of the in vitro culture.[PUBLICATION ABSTRACT]