Involvement of the REV3 gene in the methylated base-excision repair system. Co-operation of two DNA polymerases, delta and Rev3p, in the repair of MMS-induced lesions in the DNA of Saccharomyces cerevisiae

The ability of four yeast DNA polymerase mutant strains to carry out the repair of DNA treated with MMS was studied. Mutation in DNA polymerase Rev3, as well as the already known mutation in the catalytic subunit of DNA polymerase delta , were both found to lead to the accumulation of single-strand...

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Bibliographic Details
Published in:Current genetics 1997-04, Vol.31 (4), p.292-301
Main Authors: Halas, A, Baranowska, H, Policinska, Z, Jachymczyk, W.J
Format: Article
Language:English
Subjects:
ADN
BASE-EXCISION REPAIR
DNA
DNA POLYMERASE DELTA
DNA POLYMERASE REV3
ENZIMAS
ENZYME
ENZYMES
GENE
GENES
MUTACION
MUTATION
POLIMERIZACION
POLYMERISATION
POLYMERIZATION
SACCHAROMYCES CEREVISIAE
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Summary:The ability of four yeast DNA polymerase mutant strains to carry out the repair of DNA treated with MMS was studied. Mutation in DNA polymerase Rev3, as well as the already known mutation in the catalytic subunit of DNA polymerase delta , were both found to lead to the accumulation of single-strand breaks, which indicates defective repair. A double-mutant strain carrying mutations in DNA polymerase delta and a deletion in the REV3 gene had a complete repair defect, both at permissive (23 degree C) and restrictive (38 degree C) temperatures, which was not observed in other pairwise combinations of tested polymerase mutants. Other polymerases are not involved in the repair of exogenous DNA methylation damage, since neither mutation in the DNA polymerase epsilon , nor deletion in the DNA polymerase IV ( beta sub(70)) gene, caused defective repair. The data obtained suggest that DNA polymerases delta and Rev3p are both necessary to perform repair synthesis in the base-excision repair of methylation damage. The results are discussed in the light of current concepts on the role of DNA polymerase Rev3 in mutagenesis.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050208