Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectrometry

Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and α-cyan...

Full description

Saved in:
Bibliographic Details
Published in:Nature biotechnology 1997-09, Vol.15 (9), p.906-909
Main Authors: Erhard, Marcel, von Döhren, Hans, Jungblut, Peter
Format: Article
Language:English
Subjects:
Agriculture
Anabaena - metabolism
Bacterial Proteins - analysis
Bioinformatics
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cyanobacteria - metabolism
Cyanophyta
Freshwater
Fundamental and applied biological sciences. Psychology
Life Sciences
Microcystins
Miscellaneous
Mission oriented research
Peptides, Cyclic - analysis
research-article
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and α-cyano-4-hydroxycinnamic acid matrix, display spectra showing predominantly the secondary metabolites including known microcystins, micropeptin, and anabaenopeptolin. A new cyclic anabaenopeptolin has been identified using the Post-Source-Decay mode. Strains of various origins can easily be typed according to their cyclic peptide production, and toxic and nontoxic algal blooms can be differentiated within minutes.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt0997-906