Detection of chromosome 2 and chromosome 7 within X-ray-or colchicine-induced micronuclei by fluorescence in situ hybridization

• Published in: Mutagenesis 1997-03, Vol.12 (2), p.55-59
• Main Authors: Wuttke, K., Streffer, C., Muller, W.-U.
• Format: Article
• Language: English
• Subjects: Aneuploidy; Biological and medical sciences; Cell Nucleus - drug effects; Cell Nucleus - genetics; Cell Nucleus - radiation effects; Chemical mutagenesis; Chromosome Banding - methods; Chromosomes, Human, Pair 2 - drug effects; Chromosomes, Human, Pair 2 - genetics; Chromosomes, Human, Pair 2 - radiation effects; Chromosomes, Human, Pair 7 - drug effects; Chromosomes, Human, Pair 7 - genetics; Chromosomes, Human, Pair 7 - radiation effects; Colchicine - toxicity; Female; Humans; In Situ Hybridization, Fluorescence - methods; Lymphocytes - drug effects; Lymphocytes - radiation effects; Medical sciences; Micronucleus Tests; Spindle Apparatus - drug effects; Toxicology; X-Rays
• ISSN: 0267-8357; 1464-3804
• DOI: 10.1093/mutage/12.2.55

The occurrence of chromosome 2 and chromosome 7 within micronuclei of binucleated lymphocytes induced by X-rays or colchicine was scored using the whole chromosome painting technique. The observed frequency of involvement in micronucleus formation was compared with the yield that would be expected theoretically, when the random participation of each chromosome is assumed. No difference was observed between the expected and observed inclusion of chromosome 2 or chromosome 7 into micronuclei after X-ray exposure (2.5 Gy). This was also the case for chromosome 2, but not for chromosome 7 after colchicine treatment (0.04/0.06 μg/ml); chromosome 7 was detected approximately 1.5 times more frequently in micronucleus formation than would be expected from the assumption of a random distribution.