Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b

Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12g/L in continuous culture and biomass productivity was enhanced up to 0.708g/L/h. The optimal temperature and pH for soluble MMO activity were 35 degree C and 6.2-6.4. The Optimal concentrations of methanol a...

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Bibliographic Details
Published in:Biotechnology letters 1996-08, Vol.18 (8), p.897-902
Main Authors: LEE, J, SONI, B. K, KELLEY, R. L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Methylosinus trichosporium
Production of selected enzymes
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Summary:Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12g/L in continuous culture and biomass productivity was enhanced up to 0.708g/L/h. The optimal temperature and pH for soluble MMO activity were 35 degree C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced. (DBO)
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00154617