Carbachol- and elevated Ca super(2+)-induced translocation of functionally active protein kinase C to the brush border of rabbit ileal Na super(+) absorbing cells

protein kinase C is involved in mediating the effects of elevated Ca super(2+) in ileal villus Na super(+) absorbing cells to inhibit NaCl absorption. The present studies were undertaken to understand the mechanism by which this occurs. The effects of carbachol and the calcium ionophore A23187, agen...

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Bibliographic Details
Published in:The Journal of clinical investigation 1991-01, Vol.88 (3), p.855-863
Main Authors: Cohen, ME, Wesolek, J, McCullen, J, Rys-Sikora, K, Pandol, S, Rood, R P, Sharp, GWG, Donowitz, M
Format: Article
Language:English
Subjects:
calcium
carbachol
protein kinase C
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Summary:protein kinase C is involved in mediating the effects of elevated Ca super(2+) in ileal villus Na super(+) absorbing cells to inhibit NaCl absorption. The present studies were undertaken to understand the mechanism by which this occurs. The effects of carbachol and the calcium ionophore A23187, agents which elevate intracellular Ca super(2+) and inhibit NaCl absorption in ileal villus cells, were studied. In conclusion, the effect of carbachol and Ca super(2+) ionophore in regulation of ileal NaCl absorption is associated with an increase in microvillus membrane diacylglycerol content and functionally active protein kinase C. The effects of both carbachol and Ca super(2+) ionophore are different on brush border and basolateral membrane distribution of protein kinase C.
ISSN:0021-9738